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Related Experiment Video

Updated: Jan 12, 2026

Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area
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Recombinant Irisin Therapy Alters Brain Transcriptome and Protects Against Ischemic Damage in Middle-Aged Rats.

Shahil H Patel1,2,3, Indy Cabeda Diaz1,2, Zhexuan Zhang4

  • 1Peritz Scheinberg Cerebral Vascular Disease Research Laboratory (CVDRL) (S.H.P., I.C.D., A.P.R.), , University of Miami, FL.

Stroke
|November 5, 2025
PubMed
Summary

Irisin therapy significantly reduced stroke-induced brain damage in rats. This myokine treatment also improved motor and cognitive functions in female rats after ischemic stroke.

Keywords:
Morris water maze testextracellular matrixfibronectin type III domainintegrinsstroke

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Area of Science:

  • Neuroscience
  • Endocrinology
  • Regenerative Medicine

Background:

  • Irisin, a myokine derived from FNDC5, influences energy metabolism and insulin sensitivity.
  • Exercise and vibration stimulate irisin release, highlighting its physiological relevance.
  • This study investigates irisin's neuroprotective and functional recovery potential in a rat stroke model.

Purpose of the Study:

  • To evaluate the therapeutic efficacy of irisin in reducing ischemic brain damage in rats.
  • To assess the impact of irisin on sensorimotor and cognitive functions post-stroke.
  • To explore the molecular mechanisms underlying irisin's effects in the ischemic brain.

Main Methods:

  • Transient middle cerebral artery occlusion was induced in adult Sprague-Dawley rats.
  • Rats received saline or varying doses of irisin post-occlusion.
  • Infarct volume, sensorimotor/cognitive functions, and cortical gene expression were analyzed.

Main Results:

  • Irisin treatment significantly reduced infarct volume in both male and female rats.
  • A dose of 0.2 μg/g irisin improved motor function and spatial learning in senescent female rats.
  • Transcriptomic analysis revealed significant alterations in gene expression, including upregulation of extracellular matrix genes and integrin αVβ5.

Conclusions:

  • Post-stroke irisin therapy modulates the brain's transcriptome.
  • Irisin enhances motor and cognitive recovery in female rats following ischemic stroke.
  • Irisin demonstrates neuroprotective effects against ischemic brain damage.