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A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method enables simultaneous detection of nine food allergens. Interlaboratory validation confirmed its accuracy and practicality for screening processed foods.

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Area of Science:

  • Analytical Chemistry
  • Food Science
  • Mass Spectrometry

Background:

  • Food allergen analysis is crucial for public health and regulatory compliance.
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) offers high sensitivity and specificity for multi-allergen detection.
  • Developing validated methods for simultaneous food allergen analysis is an ongoing research area.

Purpose of the Study:

  • To develop and validate a simultaneous food allergen detection method using LC-MS/MS.
  • To evaluate the method's effectiveness through an interlaboratory validation study.
  • To assess the method's applicability for screening processed foods for multiple allergens.

Main Methods:

  • Development of a simultaneous detection method utilizing LC-MS/MS.
  • Application of the method to model processed foods (rice porridge, pancake).
  • Interlaboratory validation study involving three independent laboratories.

Main Results:

  • The developed LC-MS/MS method successfully targeted nine common food allergens.
  • Qualitative and quantitative analyses demonstrated good accuracy across participating laboratories.
  • High agreement was observed between laboratories, indicating method robustness, with minor exceptions for specific allergens.

Conclusions:

  • The developed LC-MS/MS method is practical and effective for the simultaneous screening of multiple food allergens.
  • The method shows potential for application in routine food safety testing and regulatory surveillance.
  • Further application to diverse food matrices is supported by the validation results.