Optimization and characterization of a lactate-oxidase electrode

Area of Science:

• Electrochemistry
• Biosensor Technology
• Biomaterials

Background:

• Electrochemical lactate sensors are vital for analyzing biological fluids in healthcare and sports.
• The performance of these sensors heavily relies on the lactate oxidase (LOx)-based electrode.

Purpose of the Study:

• To enhance the performance of lactate oxidase electrodes.
• To optimize the loading and layering of lactate oxidase (LOx) and poly(ethylene glycol) diglycidyl ether (PEGDGE) on carbon paper.

Main Methods:

• Utilized the Box-Behnken design for optimization.
• Characterized the electrode surface using FE-SEM, FTIR, and impedance analyses.
• Evaluated performance through electrochemical and biochemical analyses.

Main Results:

• The optimized electrode (4 layers LOx, 184 μg PEGDGE) achieved an oxidation current of 1840 ± 60 μA.
• Demonstrated high enzyme activity, a maximum current at 50 mM lactate, and an apparent K^app_m of 11.4 mM.
• Exhibited high stability and robust enzyme binding for repeated use.

Conclusions:

• Optimized LOx/PEGDGE loading and layering significantly enhance electrode performance.
• The developed electrode is suitable for creating more effective and dependable lactate biosensors.
• This work contributes to advancements in electrochemical biosensing for biological fluid analysis.