Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

17.1K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.1K
Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

110.4K
Overview
110.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Framework Nucleic Acids: Innovative Tools for Cellular Sensing and Therapeutics.

Chembiochem : a European journal of chemical biology·2024
Same author

NETosis-Inspired Cell Surface-Constrained Framework Nucleic Acids Traps (FNATs) for Cascaded Extracellular Recognition and Cellular Behavior Modulation.

Angewandte Chemie (International ed. in English)·2024
Same author

ELP3 stabilizes c-Myc to promote tumorigenesis.

Journal of molecular cell biology·2023
Same author

Digital image processing realized by memristor-based technologies.

Discover nano·2023
Same author

EGR1 Promotes Ovarian Hyperstimulation Syndrome Through Upregulation of SOX9 Expression.

Cell transplantation·2023
Same author

The application of HER2 and CD47 CAR-macrophage in ovarian cancer.

Journal of translational medicine·2023

Related Experiment Video

Updated: Jan 12, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.0K

Extracellularly Activated Logic-Gated DNA Nanodevice for Molecular Transport via Reprogrammed Transferrin Receptor

Peng-Fei Dai1, Hong-Wei Si1, Yu Cao2

  • 1Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China.

Small (Weinheim an Der Bergstrasse, Germany)
|November 6, 2025
PubMed
Summary

This study presents a DNA nanodevice that reprograms cancer cell transferrin receptor (TfR) trafficking to disrupt iron metabolism and inhibit cancer cell migration. It also delivers a photosensitizer for dual inhibition of cancer growth and motility.

Keywords:
DNA logic gateDNA nanotechnologyframework nucleic acidsmolecular transporttransferrin receptor

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.8K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

12.1K

Related Experiment Videos

Last Updated: Jan 12, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.0K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.8K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

12.1K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cancer Biology

Background:

  • Overexpressed transferrin receptors (TfR) on cancer cells are crucial for iron uptake.
  • Cancer cells exhibit elevated iron demand to support rapid proliferation.
  • TfR recycling is essential for maintaining iron homeostasis in cancer cells.

Purpose of the Study:

  • To develop an extracellularly activated DNA nanodevice for cancer therapy.
  • To hijack and reprogram TfR trafficking to disrupt cancer cell iron metabolism.
  • To achieve dual inhibition of cancer cell growth and motility.

Main Methods:

  • An "AND" logic-gated DNA nanodevice was designed for extracellular activation.
  • The nanodevice dynamically assembles in response to acidic pH and molecular cues.
  • Tetrahedral framework nucleic acid (tFNA)-mediated targeting captures TfRs and redirects them to lysosomes.
  • Chlorin e6 (Ce6) photosensitizer was incorporated for photodynamic therapy.

Main Results:

  • The nanodevice successfully reprogrammed TfR trafficking, redirecting it to lysosomal degradation.
  • Disruption of iron homeostasis impaired cancer cell migration.
  • Efficient intracellular delivery of Ce6 induced cytoplasmic perturbations and self-destruction.
  • Dual inhibition of cancer cell growth and motility was achieved.

Conclusions:

  • The developed DNA nanodevice demonstrates intelligent cell-level recognition for targeted cancer therapy.
  • This platform holds potential for extracellular molecular computation and precision nanomedicine.
  • Reprogramming TfR trafficking offers a novel strategy for cancer treatment.