Graphene Oxide-Based Acetamiprid Detection Platform and the Construction of Logic Gate

Area of Science:

• Analytical Chemistry
• Biotechnology
• Food Science

Background:

• Pesticides are vital for agriculture but necessitate robust residue detection methods.
• Acetamiprid, a widely used neonicotinoid insecticide, requires sensitive monitoring in food production.
• Existing pesticide residue detection technologies need advancement to meet safety standards.

Purpose of the Study:

• To develop and report a novel fluorescent biosensor for acetamiprid determination.
• To utilize graphene oxide and G-quadruplex DNA structures for enhanced detection.
• To provide a sensitive and efficient method for acetamiprid residue analysis in food safety applications.

Main Methods:

• Designed a DNA probe incorporating an acetamiprid aptamer and G-quadruplex forming sequence.
• Utilized graphene oxide for probe stabilization via pi-pi interactions, influencing assay signals.
• Employed bioinformatics tools (NUPACK, AutoDock) for sequence optimization and simulation prior to experimentation.

Main Results:

• The developed fluorescent biosensor successfully detected acetamiprid.
• Achieved a low detection limit of 165.5 pM for acetamiprid.
• The detection limit is significantly lower than the national food safety standard (0.05 mg/kg).

Conclusions:

• The graphene oxide-G-quadruplex based fluorescent biosensor is a sensitive and effective tool for acetamiprid detection.
• The sensor offers advantages including economic viability, simplicity, and speed, with significant potential for food safety monitoring.
• This technology contributes to updating pesticide residue detection, supporting agricultural and food safety regulations.