In Vitro and In Silico Studies on Unique Key Components of Hypericum Species, Focusing on Their Role in Inhibiting...

Area of Science:

• Phytochemistry
• Pharmacology
• Natural Product Chemistry

Background:

• Hypericum L. species are increasingly recognized for their therapeutic applications.
• Understanding the chemical composition and biological activities of these plants is crucial for validating their traditional uses.

Purpose of the Study:

• To investigate the chemical profiles of Hypericum empetrifolium subsp. empetrifolium, Hypericum triquetrifolium, and Hypericum pruinatum.
• To evaluate the antioxidant and various enzyme inhibitory activities of their ethanol extracts and major constituents.

Main Methods:

• Phenolic content determined using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
• In vitro antioxidant assays (CUPRAC, DPPH, ABTS) and enzyme inhibition assays (anticholinesterase, urease, tyrosinase, elastase, collagenase, ACE) were performed.
• In silico analyses were conducted for key compounds.

Main Results:

• LC-MS/MS identified significant levels of hyperoside, hesperidin, chlorogenic acid, and others in the extracts.
• Extracts demonstrated strong antioxidant potential.
• Hesperidin and quercetin exhibited substantial enzyme inhibitory activities against urease, tyrosinase, elastase, collagenase, and ACE.

Conclusions:

• Hypericum species are rich sources of bioactive compounds with notable antioxidant and enzyme inhibitory effects.
• The findings support the potential use of these Hypericum species and their components in the food and pharmaceutical sectors.