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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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CTRL Enables Gene-Specific RNA Regulation Using CRISPR-Cas7-11.

Tianqi Wang1, Daniel J Brogan1, Natalie M Zawalick2

  • 1School of Biological Sciences, Department of Cell and Developmental Biology, University of California, San Diego, La Jolla, California 92093, United States.

ACS Synthetic Biology
|November 7, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed CRISPR-Cas Transgenic Repressible eLement (CTRL), a novel gene repression tool. This synthetic biology innovation offers precise control over gene expression in various model systems.

Keywords:
CRISPR-CasCRISPR-Cas engineeringCas7–11RNA-targetinggRAMPgene circuitgene expressiongene regulationsynthetic biology

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Area of Science:

  • Synthetic biology
  • Molecular biology
  • Gene expression regulation

Background:

  • Synthetic biology advancements enable precise control over biological networks.
  • Existing gene expression technologies require refinement for comprehensive network investigations.

Purpose of the Study:

  • To develop and characterize a novel, efficient, and tunable gene repression tool.
  • To demonstrate the site-specific targeting of synthetic mRNA molecules using a CRISPR-Cas7-11 effector.

Main Methods:

  • Development of the CRISPR-Cas Transgenic Repressible eLement (CTRL) system.
  • Utilizing the direct repeat processing activity of the CRISPR-Cas7-11 effector.
  • Engineering multiple permutations of the Cas7-11 effector for varied repression efficiencies.

Main Results:

  • CTRL demonstrates high efficiency in gene repression.
  • The system offers tunable regulation of gene expression.
  • CTRL achieves gene-specific repression at both mRNA and protein levels.
  • Engineered Cas7-11 variants show differential gene silencing capabilities.

Conclusions:

  • CTRL is a versatile and innovative tool for gene repression technology.
  • The system exhibits broad applicability across diverse model organisms.
  • CTRL provides flexibility for researchers in controlling gene expression with precision.