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Biophysical and structural studies on transketolases.

Fabian Rabe von Pappenheim¹, Kai Tittmann¹

¹Department of Molecular Enzymology, Göttingen Center of Molecular Biosciences and Albrecht-von-Haller Institute, Georg-August University Göttingen, Göttingen, Germany; Max-Planck-Institute for Multidisciplinary Sciences, Göttingen, Germany.

Methods in Enzymology

|November 7, 2025

Summary

This study details methods for analyzing transketolase, a crucial enzyme in various fields. Understanding its structure and mechanism aids in drug development and disease treatment.

Keywords:

Cofactor Enzymatic assay Enzyme mechanism Intermediates Kinetics Spectroscopy Stopped-flow Thiamin diphosphate

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Published on: May 13, 2020

Area of Science:

Biochemistry and enzymology
Synthetic biology and biocatalysis
Drug discovery and disease mechanisms

Background:

Transketolase (TK) is vital for thiamin-dependent chemistry and has implications in cancer, metabolic, and neurodegenerative diseases.
Understanding TK's mechanism and structure is key for designing enzyme variants and novel biosynthetic pathways.
TK research spans enzymology, synthetic biology, drug development, and biocatalysis.

Purpose of the Study:

To present comprehensive protocols for analyzing transketolase activity, mechanism, and structure.
To provide a toolkit for advancing fundamental and applied research on transketolase.
To enable rational design of transketolase variants and creation of new biosynthetic pathways.

Main Methods:

Steady-state kinetics assays to determine enzyme activity.
Cofactor binding studies to understand enzyme-substrate interactions.
Spectroscopic and biophysical techniques for detecting catalytic intermediates and rapid kinetic studies.

Main Results:

Established protocols for comprehensive transketolase analysis.
Enabled detailed investigation of enzyme catalysis and thiamin-dependent chemistry.
Provided methods for characterizing transketolase structure and function.

Conclusions:

The presented protocols offer a robust toolkit for transketolase research.
This work facilitates advancements in enzyme engineering and biocatalysis.
Understanding transketolase is crucial for therapeutic target identification and pathway engineering.