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Oxidative Stress-Related HDL Dysfunction in Hemodialysis: The Clinical Utility of MPO/PON1 and MPO/HDL-C Ratios in
Elif Azize Özşahin Delibaş1, Kader Köse2,3, Cevat Yazici2,3
1Faculty of Health Sciences, Department of Nutrition and Dietetics, Tokat Gaziosmapasa University, Tokat, Turkey.
Insights
The MPO/PON1 ratio may serve as a novel biomarker for oxidative stress and cardiovascular disease risk in hemodialysis patients. Evaluating HDL functionality can improve risk stratification for these high-risk individuals.
Area of Science:
- Nephrology
- Cardiology
- Biochemistry
Background:
- Chronic kidney disease (CKD) patients on hemodialysis (HD) face elevated cardiovascular disease (CVD) risk due to oxidative stress (OS), inflammation, and lipoprotein dysfunction.
- Key OS biomarkers include myeloperoxidase (MPO), paraoxonase-1 (PON1), and homocysteine (HCY).
Purpose of the Study:
- To investigate the association between OS biomarkers (MPO, PON1, HCY) and their derived ratios with cardiovascular risk in HD patients.
- To explore the potential of MPO/PON1 and other ratios as novel biomarkers for CVD risk in this population.
Main Methods:
- A case-control study involving 42 HD patients and 42 matched healthy controls.
- Measurement of plasma HCY, MPO activity, PON1 activity, and lipid profiles.
- Calculation of functional OS ratios, including MPO/PON1 and MPO/HDL-C.
Main Results:
- HD patients exhibited significantly higher MPO activity and HCY levels, alongside lower PON1 activity compared to controls (p < 0.001).
- Elevated MPO/PON1 and MPO/HDL-C ratios were observed in HD patients both before and after dialysis, indicating heightened oxidative imbalance.
- These findings suggest a significant link between OS biomarkers and increased cardiovascular risk in HD patients.
Conclusions:
- The MPO/PON1 ratio emerges as a potential novel biomarker for assessing OS and CVD risk in HD patients.
- Evaluating HDL functionality alongside traditional biomarkers may enhance cardiovascular risk stratification in CKD patients undergoing HD.
Introduction:
Oxidative stress (OS), inflammation, and lipoprotein dysfunction contribute to the high cardiovascular disease (CVD) risk in chronic kidney disease (CKD) patients undergoing hemodialysis (HD). This study examined whether OS-related biomarkers-myeloperoxidase (MPO), paraoxonase-1 (PON1), and homocysteine (HCY)-and their derived ratios (MPO/PON1, MPO/HDL-C, PON1/HDL-C) are associated with cardiovascular risk.
Methods:
A case-control study was conducted including 42 HD patients and 42 matched healthy controls. Plasma HCY levels, MPO activity, and PON1 activity were measured, along with lipid profiles. Functional OS ratios were calculated.
Results:
HD patients showed higher MPO activity and HCY levels and lower PON1 activity compared with controls (p < 0.001). MPO/PON1 and MPO/HDL-C ratios were significantly elevated before and after dialysis, indicating increased oxidative imbalance.
Conclusion:
The MPO/PON1 ratio may represent a novel biomarker of OS and CVD risk in HD patients, supporting the evaluation of HDL functionality for improved risk stratification.
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