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In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
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Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
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Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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Data Stitching for Dynamic Field Monitoring With NMR Probes.

Jinyuan Zhang1,2,3, Zihao Zhang1,2,3, Zhentao Zuo1,3

  • 1State Key Laboratory of Cognitive Science and Mental Health, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Magnetic Resonance in Medicine
|November 9, 2025
PubMed
Summary

A new stitching method accurately characterizes magnetic field dynamics in MRI sequences, enabling higher resolution imaging. This technique works even when standard methods fail, improving image reconstruction at ultrahigh fields.

Keywords:
2D spiraldynamic field monitoringhigh‐order MR simulationhigh‐order image reconstructionspiral imaging

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Area of Science:

  • Magnetic Resonance Imaging (MRI)
  • Biophysics
  • Medical Physics

Background:

  • Standard field monitoring in MRI is limited by resolution and readout length.
  • Characterizing dynamic magnetic fields is crucial for advanced MRI sequences.

Purpose of the Study:

  • To propose a novel stitching method for characterizing MRI sequences with enhanced resolution and readout length.
  • To overcome limitations of standard field monitoring approaches.

Main Methods:

  • A stitching method combining segment-specific dynamic field measurements across consecutive TRs.
  • Demonstrated using 2D spiral sequences at 10.5T and 7T with simulated and experimental MRI data.
  • Validated in human brain imaging at 7T and compared against gradient impulse response function (GIRF) prediction.

Main Results:

  • The stitching method outperformed standard approaches at 10.5T, providing accurate dynamic field measurements.
  • At 7T, it yielded comparable results to standard methods where applicable and sensible measurements where standard methods failed.
  • Improved image reconstruction for both simulated and experimental MRI data at 7T.

Conclusions:

  • The proposed stitching method effectively characterizes challenging imaging gradients using commercial hardware.
  • It does not assume a linear gradient system, offering broad utility for ultrahigh-resolution MRI at ultrahigh fields.