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Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

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Polyphenol-Mediated Antibody Functionalization of Titanium Peroxide Nanoparticles for Cancer Cell Targeting.

Hiroaki Akasaka¹, Makiko Nakahana¹, Masao Nakayama²

¹Division of Radiation Oncology, Graduate School of Medicine, Kobe University, 7-5-2 Kusunokicho, Chuo-ku, Kobe 650-0017, Japan.

ACS Applied Bio Materials

|November 10, 2025

Summary

Researchers developed a simple method to attach antibodies to nanoparticles using tannic acid. This targeted nanoparticle delivery enhances cancer cell binding and improves radiotherapy efficacy for CD44-positive tumors.

Keywords:

drug delivery systems metal−phenolic networks nanoparticles phenolics radiation sensitizer radiotherapy

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Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis

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Published on: October 26, 2015

Area of Science:

Biotechnology
Nanomedicine
Materials Science

Background:

Efficiently functionalizing nanoparticles with targeting ligands like antibodies is crucial for biotechnological applications, including cancer therapy.
Poly(acrylic acid)-modified titanium peroxide NPs (PAATiO𝑥) show radiosensitizing effects but lack tumor-specific accumulation.
Targeting ligands are needed to improve nanoparticle delivery and therapeutic outcomes.

Purpose of the Study:

To develop a simple, one-pot method for noncovalently grafting antibodies onto nanoparticles.
To enhance the targeting and therapeutic efficacy of poly(acrylic acid)-modified titanium peroxide NPs (PAATiO𝑥) for cancer treatment.
To evaluate the performance of antibody-functionalized nanoparticles in vitro and in vivo.

Main Methods:

A one-pot, polyphenol-mediated strategy using tannic acid to graft anti-CD44 antibodies onto PAATiO𝑥 NPs.
Evaluation of cellular binding and internalization of targeted NPs in CD44-expressing cancer cell lines (MIAPaCa-2, MDA-MB-231).
Assessment of radiosensitizing efficacy and in vivo biodistribution and tumor accumulation of targeted NPs.

Main Results:

Antibody-functionalized NPs showed significantly enhanced binding (∼2-fold) and cellular internalization (2.4-fold in MIAPaCa-2, 6.5-fold in MDA-MB-231) compared to unmodified NPs.
Targeted NPs maintained radiosensitizing properties, inhibiting CD44-expressing cell growth by 2-fold compared to CD44-negative cells.
In vivo studies demonstrated approximately 2-fold greater tumor accumulation of targeted NPs in tumors compared to unmodified NPs (p < 0.05).

Conclusions:

Polyphenol-mediated antibody coating is a versatile platform for enhancing nanoparticle delivery to specific cell populations.
This strategy significantly improves nanoparticle targeting, cellular uptake, and therapeutic efficacy in CD44-positive cancer models.
The developed method holds potential for improving radiotherapy outcomes in CD44-positive tumors.