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Induced Pluripotent Stem Cells (iPSC) and Their Use in Disease Modeling
Alicja Dorota1, Nicole Maryniak1, Anna Mariankowska2
1Medical School, Zagłębiowskie Oncology Center, Dąbrowa Górnicza, POL.
Cureus
|November 10, 2025
Summary
Induced pluripotent stem cells (iPSCs) offer powerful tools for disease modeling and regenerative medicine by differentiating into patient-specific cells. However, iPSC culture presents challenges like genomic instability and requires strict quality control for clinical applications.
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Disease Modeling
Background:
- Induced pluripotent stem cells (iPSCs) are derived from somatic cells, offering patient-specific cell types.
- Reprogramming typically involves overexpressing Oct4, Sox2, Klf4, and c-Myc transcription factors.
- iPSCs hold promise for in vitro disease modeling and therapeutic applications.
Purpose of the Study:
- To review iPSC culturing methodologies.
- To highlight iPSC applications in disease modeling and regenerative medicine.
- To discuss challenges and quality control measures in iPSC technology.
Main Methods:
- Review of existing literature on iPSC reprogramming and culture.
- Analysis of iPSC differentiation capabilities for disease modeling.
- Examination of quality control techniques for iPSC maintenance.
Main Results:
- iPSCs can be generated from various somatic cells (keratinocytes, fibroblasts, etc.).
- Patient-specific iPSCs enable modeling of diverse disorders (neurodegenerative, cardiovascular, etc.).
- iPSC culture requires controlled conditions due to genomic instability.
Conclusions:
- iPSC technology advances personalized medicine, pathogenesis studies, and drug screening.
- Challenges include genomic instability, clinical safety, and standardization of culture protocols.
- Rigorous quality control is crucial for reliable iPSC applications.
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