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Related Experiment Video

Updated: Jan 11, 2026

Intravital Widefield Fluorescence Microscopy of Pulmonary Microcirculation in Experimental Acute Lung Injury Using a Vacuum-Stabilized Imaging System
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Golgi-Targeted Viscosity Fluorescent Probe for Monitoring Acute Lung Injury.

Yaqian Li1, Zi Yang1, Jieni Lei1

  • 1Hunan Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, Hunan Provincial University Key Laboratory of the Fundamental and Clinical Research on Functional Nucleic Acid, The First Clinical College of Changsha Medical University, Changsha, 410219, P. R. China.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|November 11, 2025
PubMed
Summary

Researchers developed GV, a new probe to measure Golgi viscosity, a key indicator in acute lung injury (ALI). Increased Golgi viscosity in ALI models suggests it could be a novel biomarker for this severe respiratory disease.

Keywords:
Acute lung injuryFluorescent probeGolgi targetedViscosity

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Area of Science:

  • Biophysics
  • Cell Biology
  • Medical Diagnostics

Background:

  • Acute lung injury (ALI) is a critical respiratory condition characterized by uncontrolled inflammation.
  • Early diagnosis and intervention are crucial for managing ALI.
  • Emerging research suggests Golgi apparatus dysfunction plays a role in ALI, but its biophysical changes are not well understood.

Purpose of the Study:

  • To investigate the biophysical changes, specifically viscosity, within the Golgi apparatus during acute lung injury.
  • To develop and validate a novel fluorescent probe for sensitive imaging of Golgi viscosity.
  • To explore the potential of Golgi viscosity as a biomarker for ALI.

Main Methods:

  • Development of GV, a novel, Golgi-targeted fluorescent probe with high photostability, low cytotoxicity, and excellent specificity.
  • Utilized cellular models to assess Golgi viscosity changes under stress conditions relevant to ALI.
  • Employed mouse models of ALI to validate findings in a complex biological system.

Main Results:

  • The novel probe GV enabled sensitive imaging of viscosity changes within the Golgi apparatus.
  • A significant increase in Golgi viscosity was observed during the progression of acute lung injury in both cellular and mouse models.
  • GV demonstrated high specificity and sensitivity in detecting these biophysical alterations.

Conclusions:

  • Golgi apparatus viscosity emerges as a potential novel biomarker for the early diagnosis and monitoring of acute lung injury.
  • The developed probe, GV, is a valuable tool for studying organelle-level biophysical changes in inflammatory diseases like ALI.
  • This study opens new avenues for understanding the role of organelle biophysics in disease pathogenesis.