Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 4, 2026

Transmitochondrial Cybrid Generation Using Cancer Cell Lines
07:49

Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

Mitochondria-Damaging Self-Reporting Probe for Cancer Therapy.

Hai Xu1, Yura Lee2, Sanghee Yoon3

  • 1Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, Republic of Korea.

Angewandte Chemie (International Ed. in English)
|June 3, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activatable smart contrast agents for photoacoustic imaging.

Smart molecules : open access·2026
Same author

Advances in BODIPY Derivatives for Antibacterial Phototherapy.

Angewandte Chemie (International ed. in English)·2026
Same author

Clinical fluorescent probes: mechanisms, advantages and inspirations.

Chemical science·2026
Same author

An Integrated Gut-on-a-Chip System for the Evaluation of Photosensitizers under Complex Microenvironmental Conditions.

Analytical chemistry·2026
Same author

A Mitochondria-Targeted Single Fluorescent Probe for Orthogonal Dual-Channel Imaging of Polarity and Viscosity during Ferroptosis.

Analytical chemistry·2026
Same author

<i>In silico</i> design and binding mechanism of UBR1 E3 ligase recruiters.

RSC advances·2026
Same journal

Efficient Syngas Photoproduction Enabled by Electronic Engineering of Co-Immobilized Imine COFs.

Angewandte Chemie (International ed. in English)·2026
Same journal

Pathway Controlled Phase Separation of Minimal Building Blocks Utilizing a Dissociative Chemical Transformation.

Angewandte Chemie (International ed. in English)·2026
Same journal

Interaction Hierarchy and Polymorphic Structure-Property Dynamics in Luminescent Molecular Crystals.

Angewandte Chemie (International ed. in English)·2026
Same journal

The Role of Zn-Hf Site Proximity and Oxygen Vacancies for Methanol Formation Over ZnHfO<sub>x</sub> Catalysts Under CO<sub>2</sub> Hydrogenation Conditions.

Angewandte Chemie (International ed. in English)·2026
Same journal

Breaking the Linear Scaling Relationship: Bioinspired Electronic Coupling in S-Bridged Fe-Fe Dual Sites for Efficient Oxygen Reduction.

Angewandte Chemie (International ed. in English)·2026
Same journal

Programming Bio-Bio Electronic Interfaces for Light-Driven Interspecies Electron Transfer.

Angewandte Chemie (International ed. in English)·2026
See all related articles
This summary is machine-generated.

New self-reporting cancer drugs (DPPs) monitor their own mitochondrial action, avoiding unreliable probes. This allows precise tracking of drug effects for improved cancer therapy and diagnosis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Chemotherapy often damages mitochondria, disrupting mitochondrial membrane potential (ΔΨm), a key factor in drug efficacy.
  • Current methods using fluorescent probes to assess ΔΨm-targeting drugs are unreliable, leading to inaccurate conclusions and hindering drug development.

Purpose of the Study:

  • To develop a novel class of self-reporting chemotherapeutic agents (DPPs) for direct, non-invasive monitoring of mitochondrial drug action.
  • To evaluate the efficacy and safety of DPPs in preclinical cancer models.

Main Methods:

  • Synthesized and characterized a series of cationic chemotherapeutic small molecules (DPPs) with intrinsic fluorescence.
  • Assessed the ability of DPPs to disrupt mitochondrial function, induce reactive oxygen species (ROS), and trigger apoptosis.
Keywords:
cancer therapyfluorescence imagingmitochondriaself‐reporting probe

More Related Videos

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
11:32

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates

Published on: August 6, 2021

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
07:58

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

Published on: May 12, 2020

Related Experiment Videos

Last Updated: Jun 4, 2026

Transmitochondrial Cybrid Generation Using Cancer Cell Lines
07:49

Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
11:32

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates

Published on: August 6, 2021

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
07:58

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

Published on: May 12, 2020

  • Investigated the mitochondrial-to-nuclear translocation of DPPs in real-time.
  • Evaluated tumor growth inhibition and systemic toxicity in vivo.

    • Main Results:

    • DPPs exhibit intrinsic fluorescence, enabling self-reporting of their action without external probes.
    • DPP-1 and DPP-2 disrupt mitochondrial function, increase ROS generation, and induce selective apoptosis.
    • Concentration-dependent mitochondrial-to-nuclear translocation of DPPs allows real-time visualization of therapeutic progression.
    • In vivo studies demonstrated potent tumor growth inhibition with negligible systemic toxicity.

    Conclusions:

    • DPPs offer a reliable platform for monitoring mitochondria-targeted chemotherapy, overcoming limitations of current fluorescent probes.
    • The self-reporting capability of DPPs facilitates real-time assessment of drug efficacy at the subcellular level.
    • This mitochondria-targeted chemotherapeutic strategy holds promise for integrated cancer diagnosis and precision therapy.