Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rapid In Vitro Pathological Diagnosis of Glioma Using Dual-Color Quantum Dot Probes for Precise Intraoperative Resection.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Integrated Multi-Omics and Experimental Validation Reveal Dysregulation of the OXPHOS-NADPH-GSH Axis in Renal Fibrosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same author

Corrigendum to "Adaptive cationic peptide bundles hydrogel for the repair of infected mandibular defects" [Biomaterials 325 (2026) 123613].

Biomaterials·2026

Same author

Printed Microscale Photonic Resonant Structured Photodetectors for Monitoring Eye Movement Abnormalities.

ACS sensors·2026

Same author

Physical activity, sedentary behavior and ovarian cancer risk in Asian populations: a scoping review and meta-analysis.

BMC cancer·2026

Same author

Peptide dendritic polymerization-enabled stable and tailorable nanoparticles as crosslinkers for fabricating multifunctional hydrogels with enhanced wound healing efficacy.

Journal of materials chemistry. B·2026

Search research articles

Related Experiment Video

Updated: Jan 11, 2026

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

Decoding the Prognosis-Related S100A9^high Monocyte in Glioblastoma Using Single-Cell and Spatial Transcriptome

Xiucan Li^1,2, Ying Qin³, Pengfei Gao²

¹Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, People's Republic of China.

Oncotargets and Therapy

|November 11, 2025

Summary

Researchers identified a novel S100A9^high monocyte subset in glioblastoma that promotes tumor growth. These monocytes create an immunosuppressive environment, correlating with poor patient prognosis and offering potential therapeutic targets.

Keywords:

glioblastoma immune environment scRNA-seq spatial transcriptome sequencing tumor associated monocyte

More Related Videos

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Published on: April 12, 2020

Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening

Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening

Published on: September 5, 2025

Related Experiment Videos

Last Updated: Jan 11, 2026

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Published on: April 12, 2020

Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening

Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening

Published on: September 5, 2025

Area of Science:

Neuro-oncology
Immunology
Single-cell genomics

Background:

Monocytes and macrophages are key immune cells in human glioblastoma, influencing tumor progression.
The heterogeneity within the monocyte population in glioblastoma is not fully understood.

Purpose of the Study:

To investigate the heterogeneity and function of monocytes in glioblastoma.
To characterize distinct glioblastoma subtypes based on ligand-receptor networks.

Main Methods:

Comprehensive analysis using single-cell RNA sequencing (scRNA-seq).
Spatial transcriptome sequencing combined with immunofluorescence assays.
In vitro T cell co-culture assays.

Main Results:

Identified three glioblastoma subtypes; the C3 subtype showed a higher proportion of S100A9^high monocytes associated with poor prognosis.
S100A9^high monocytes were spatially located near M2 macrophages, exhausted CD8+ T cells, and endothelial cells.
S100A9^high monocytes produced immunosuppressive IL-10, ROS, and iNOS, potentially impairing T cell function.

Conclusions:

Deciphered monocyte heterogeneity in glioblastoma.
Identified S100A9^high monocytes as a novel subset linked to poor prognosis.
These monocytes contribute to an immunosuppressive, pro-tumorigenic microenvironment in glioblastoma.