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Updated: Jan 11, 2026
Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
Published on: May 18, 2021
Generation and characterization of novel high-affinity fully human antibodies targeting prostate-specific membrane
Claudia Comacchio1, Stefanie K Pfister1, Domenico Ravazza1
1Department of Biology, Philochem AG, Libernstrasse 3, 8112 Otelfingen (ZH), Switzerland.
Background:
Prostate-specific membrane antigen (PSMA)-targeted therapies have revolutionized the management of patients with prostate cancer. However, not all patients benefit from such agents. Prostatic acid phosphatase (ACP3), a tyrosine phosphatase produced by prostate epithelial cells, has emerged as a promising alternative target for both early and advanced disease.
Methods:
Here, we report on the generation and characterization of novel, fully human monoclonal antibodies targeting PSMA and ACP3. Antigen-specific clones were isolated from human antibody phage display libraries and characterized in vitro and in vivo.
Results:
Selected clones bound with high specificity to the cognate target and demonstrated excellent tumor-targeting performance in biodistribution studies.
Conclusions:
The lead candidates, termed H92A5 (anti-PSMA) and EKA4 (anti-ACP3), could serve as promising scaffolds for the development of next-generation tumor-targeted therapeutics for prostate cancer.
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