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Related Concept Videos

Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
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Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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Related Experiment Video

Updated: Jun 14, 2026

Extraction of Extracellular Vesicles from Whole Tissue
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Recommendations for Studying In Situ Extracellular Vesicles From Solid Tissue.

Rossella Crescitelli1, Yiyao Huang2,3, An Hendrix4,5

  • 1Sahlgrenska Center for Cancer Research, Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Journal of Extracellular Vesicles
|November 12, 2025
PubMed
Summary
This summary is machine-generated.

Solid tissue-derived extracellular vesicles (ST-EVs) offer insights into tissue health but are challenging to isolate. Standardized reporting is vital for reproducible ST-EV research and clinical applications.

Keywords:
characterizationexosomesextracellular vesiclesguidelinesisolation reproducibilityseparationtissue

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Area of Science:

  • Extracellular Vesicle Biology
  • Biotechnology
  • Tissue Engineering

Background:

  • Solid tissue-derived extracellular vesicles (ST-EVs) reflect tissue-specific molecular states.
  • Isolating ST-EVs presents unique technical challenges compared to biofluid-derived EVs.
  • Reproducibility and comparability in ST-EV research are hindered by inconsistent methodologies.

Purpose of the Study:

  • To highlight the importance of transparent reporting in solid tissue-derived extracellular vesicle (ST-EV) research.
  • To recommend reporting parameters for pre-analytical and analytical handling of solid tissues for ST-EV isolation.
  • To identify key challenges and future directions in ST-EV separation and characterization.

Main Methods:

  • Literature review and expert consensus from the International Society for Extracellular Vesicles' Solid Tissue Task Force.
  • Analysis of current methodologies for ST-EV enrichment and characterization.
  • Identification of critical parameters for transparent reporting in ST-EV studies.

Main Results:

  • ST-EVs offer a physiologically relevant snapshot of tissue molecular dynamics.
  • Standardized reporting is crucial for enhancing the reproducibility and comparability of ST-EV research.
  • Specific pre-analytical and analytical parameters require careful consideration for reliable ST-EV isolation.

Conclusions:

  • Advancing the understanding of ST-EV biological roles requires standardized research practices.
  • Addressing technical challenges in ST-EV enrichment is essential for reliable data.
  • Implementing transparent reporting guidelines will facilitate the clinical application of ST-EVs.