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Related Experiment Video

Updated: May 12, 2026

Continuous Flow Chemistry: Reaction of Diphenyldiazomethane with p-Nitrobenzoic Acid

Continuous Flow Chemistry: Reaction of Diphenyldiazomethane with p-Nitrobenzoic Acid

Published on: November 15, 2017

Accelerating innovation in peptide synthesis through continuous-flow.

Shinichiro Fuse¹

¹Graduate School of Pharmaceutical Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan. fuse.shinichiro.z3@f.mail.nagoya-u.ac.jp.

Organic & Biomolecular Chemistry

|November 12, 2025

Summary

Continuous-flow synthesis enhances peptide production, offering greener, faster, and more cost-effective methods for pharmaceuticals and materials. Recent advancements focus on efficiency and sustainability in peptide chain elongation and coupling.

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Last Updated: May 12, 2026

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Published on: November 15, 2017

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An Inexpensive Adaptation of a Commercial Microwave Reactor for Solid Phase Peptide Synthesis

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Published on: November 22, 2024

Area of Science:

Medicinal Chemistry
Organic Synthesis
Chemical Engineering

Background:

Over 110 peptide-based drugs are approved, with growing applications in agrochemicals and materials science.
Existing peptide synthesis methods face challenges in waste generation, reaction time, cost, and environmental impact.
Continuous-flow synthesis offers a promising alternative for efficient and sustainable chemical production.

Purpose of the Study:

To review recent advancements in continuous-flow peptide synthesis over the past four years.
To highlight strategies addressing waste reduction, efficiency, cost-effectiveness, and environmental impact.
To discuss current challenges and future perspectives in the field.

Main Methods:

Review of recent literature (last four years) on continuous-flow peptide synthesis.
Categorization of advancements into solid-phase and solution-phase peptide chain elongation, cyclization, and coupling.
Analysis of strategies for waste minimization, time reduction, cost-effectiveness, and environmental sustainability.

Main Results:

Continuous-flow synthesis enables rapid production of peptides exceeding 200 residues.
One-flow multi-component coupling facilitates rapid peptide chain elongation.
Good Manufacturing Practice (GMP)-compliant kilogram-scale peptide production is achievable.
Various strategies have been developed to mitigate waste, time, cost, and environmental concerns.

Conclusions:

Continuous-flow synthesis has revolutionized peptide production, offering significant improvements in efficiency and sustainability.
Ongoing research focuses on further optimizing these methods for diverse applications.
Future work should address remaining challenges to fully realize the potential of flow chemistry in peptide synthesis.