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Updated: Jul 31, 2026
Growth and Characterization of Irradiated Organoids from Mammary Glands
Published on: May 3, 2019
Dose response test of patient-derived cancer organoids to irradiation
Haina Yu1, Canfeng Lin2, Fan Chen3
1Department of Medical Oncology, Jinjiang Municipal Hospital (Shanghai Sixth People's Hospital Fujian), Quanzhou, China.
Background:
Patient-derived cancer organoids emerged as an innovative model in basic and translational medicine research, as well as precision medicine. However, most radiation oncologists are still unaware of the value of organoids in radiation oncology research, especially in precision radiation oncology. The methods for assessing organoid cell death after irradiation are also undefined.
Methods:
Three organoid lines were successfully established from the surgical specimens of rectal cancer patients. Genetic characterization of rectal cancer organoids was conducted. The survival of rectal cancer organoids after X rays irradiation was evaluated by image-based analysis and cell counting kit-8.
Results:
The three organoid lines displayed diverse architecture and captured heterogeneity of the tumors. The most frequently mutated genes and pathways among rectal cancer tumors also presented in rectal cancer organoids. Image-based analysis validated organoid survival after X rays irradiation, while cell counting kit-8 failed. The survival curves from imaging analysis were more representative, with an initial linear slope, followed by a shoulder, and tending to become straight again. This was the first study to investigate the appropriate testing method of using organoids to study cancer radiosensitivity.
Conclusion:
Cancer organoids were easy to use for the research of radiobiology of cancer. Image-based analysis is more accurate than cell viability (tested by cell counting kit-8) for studying cancer radiosensitivity via organoids.
Insights
Patient-derived cancer organoids offer a valuable tool for radiation oncology research. Image-based analysis is a more accurate method than cell counting kit-8 for assessing cancer organoid radiosensitivity.
Area of Science:
- Oncology
- Radiotherapy Research
- Cancer Biology
Background:
- Patient-derived cancer organoids are innovative models for precision medicine.
- Radiation oncologists need to recognize the value of organoids in radiation oncology.
- Standardized methods for assessing organoid cell death post-irradiation are lacking.
Purpose of the Study:
- To establish and characterize rectal cancer organoid lines.
- To evaluate methods for assessing rectal cancer organoid radiosensitivity.
- To determine the most accurate method for evaluating organoid survival after irradiation.
Main Methods:
- Established three rectal cancer organoid lines from surgical specimens.
- Performed genetic characterization of the organoid lines.
- Assessed organoid survival after X-ray irradiation using image-based analysis and Cell Counting Kit-8.
Main Results:
- Organoid lines reflected tumor heterogeneity and common mutations.
- Image-based analysis accurately validated organoid survival post-irradiation.
- Cell Counting Kit-8 failed to accurately assess survival; imaging provided more representative survival curves.
Conclusions:
- Cancer organoids are practical for radiobiology research.
- Image-based analysis is superior to Cell Counting Kit-8 for studying cancer organoid radiosensitivity.
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