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Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
Published on: August 31, 2021
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Protocol to quantify mechanical properties of cells using optical tweezers
Wessel S Rodenburg1, Jorine M Eeftens1
1Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6523 AJ Nijmegen, the Netherlands; Radboud Institute for Molecular Life Sciences, Radboud University, Geert Grooteplein-Zuid 28, 6525 GA Nijmegen, the Netherlands.
STAR Protocols
|November 12, 2025
Summary
This study presents a protocol to measure cell mechanical properties using optical tweezers to apply piconewton forces. This method quantifies cell deformation, crucial for understanding cellular functions.
Area of Science:
- Biophysics
- Cell Biology
- Biomechanical Engineering
Background:
- Cellular mechanical properties, such as deformability, are vital for numerous biological processes.
- Quantifying these properties in living cells is essential for understanding cell behavior and function.
Purpose of the Study:
- To present a detailed protocol for quantifying the mechanical properties of living cells.
- To enable researchers to measure cell deformability under controlled force application.
Main Methods:
- Utilizing optically trapped microspheres to apply piconewton-range forces to cells.
- Describing sample preparation for both adherent and suspended cells.
- Detailing optical trap calibration and force-deformation curve generation for data analysis.
Main Results:
- The protocol allows for the precise quantification of cellular mechanical properties.
- Force-deformation curves are generated to analyze cell response to applied forces.
- The method is applicable to various cell types, including adherent and suspended cells.
Conclusions:
- The presented protocol offers a robust method for measuring living cell mechanical properties.
- Accurate quantification of cell deformability is achievable using optical tweezers.
- This technique provides valuable insights into cell mechanics for biological research.
