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Microscale assay for the quantification of total polysaccharides to estimate extracellular polymeric substances (EPS)
G Bogar1, J T Lennon2, H M Vander Stel3
1Department of Plant Biology, University of California, Davis, 1002 Green Hall, Davis, CA 95616, USA.
Journal of Microbiological Methods
|November 12, 2025
Abstract:
We present an inexpensive and practical microscale spectrophotometric acid-phenol assay to quantify polysaccharides in aqueous solution. The instant reaction within disposable materials is designed and benchmarked for use in quantifying extracellular polymeric substances (EPS) in soils. Precipitation recovery was high across expected ranges of soil EPS content.
