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Updated: Jan 11, 2026

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

Advances in Lycopene Production: From Natural Sources to Microbial Synthesis Using Yarrowia lipolytica.

Paweł Moroz¹, Aleksandra Bartusiak¹, Julia Niewiadomska¹

¹Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37, 51-630 Wroclaw, Poland.

Molecules (Basel, Switzerland)

|November 13, 2025

Summary

This study engineered yeast for sustainable lycopene production using short-chain fatty acids. Enhanced phospholipid biosynthesis in the yeast boosted lycopene yield, offering a promising alternative to traditional methods.

Keywords:

Yarrowia lipolytica lycopene phospholipids short-chain fatty acids

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Last Updated: Jan 11, 2026

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

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Light-Controlled Fermentations for Microbial Chemical and Protein Production

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Published on: March 22, 2022

Area of Science:

Metabolic Engineering
Synthetic Biology
Biotechnology

Background:

Lycopene, a potent antioxidant carotenoid, is valuable for food, pharmaceutical, and cosmetic industries.
Conventional lycopene production faces challenges like low yields, high costs, and environmental impact.
Microbial cell factories offer a sustainable alternative for carotenoid biosynthesis.

Purpose of the Study:

To engineer the oleaginous yeast *Yarrowia lipolytica* for efficient lycopene production.
To utilize inexpensive, renewable short-chain fatty acids (SCFAs) as carbon sources.
To investigate the role of co-activating phospholipid and carotenoid biosynthesis pathways.

Main Methods:

Engineered *Yarrowia lipolytica* by integrating four heterologous genes (*crtI*, *crtB*, *crtE*, *idi*) from *Pantoea agglomerans*.
Utilized codon optimization and Golden Gate Assembly for gene integration.
Cultivated engineered strains on SCFA media (acetate, butyrate, propionate).

Main Results:

Achieved a high lycopene yield of 462.9 mg/g dry cell weight and a titer of 3.41 g/L using butyrate.
Identified a strain with enhanced phospholipid biosynthesis (PS05/4lyc/GGA) exhibiting superior lycopene accumulation.
Demonstrated that SCFA utilization enhances acetyl-CoA availability and redirects flux towards the mevalonate pathway.

Conclusions:

Co-activation of phospholipid and carotenoid biosynthesis pathways is crucial for efficient lycopene accumulation in yeast.
Engineered *Y. lipolytica* using SCFAs provides a sustainable and high-yield platform for lycopene production.
Elevated membrane lipid biosynthesis improves metabolic stability and stress tolerance in engineered strains.