Plasma Fractionation and Mixture Improves Coverage in Proteomic Analysis.

Area of Science:

• Proteomics
• Biochemistry

Background:

• Plasma proteome coverage is crucial for blood biomarker discovery.
• Current methods have limitations in identifying the full spectrum of plasma proteins.

Purpose of the Study:

• To develop an efficient strategy for expanding plasma proteome coverage.
• To enhance the identification of proteins in plasma samples.

Main Methods:

• Sequential precipitation of plasma using increasing concentrations of acetonitrile (AcN).
• Fractionation of proteins using polyethylene glycol (PEG) precipitation and albumin depletion.
• Combining specific fractions from AcN and PEG precipitation for a mixed sample.

Main Results:

• Identification of 5441 proteins, a significant increase from 2040 proteins detected in whole plasma.
• Demonstrated the effectiveness of the fractionation and mixture strategy.
• Generated one of the largest plasma proteomics datasets.

Conclusions:

• The proposed fractionation and mixture strategy is highly effective for expanding plasma proteome coverage.
• This approach offers an efficient method for comprehensive plasma proteomics.
• The findings support the use of this strategy for enhanced blood biomarker discovery.