Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

680
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
680
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.0K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.0K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

2.1K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2.1K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.3K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.3K
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

1.7K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
1.7K
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

2.9K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic test accuracies of 4AT items are consistent across the range of baseline cognition: results from two prospective studies.

Age and ageing·2026
Same author

Enhancing Biogenic Formic Acid Production in the Modified OxFA Process by Acetonitrile Addition.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Advancing produced water quality via integrated mechanical vapor recompression: Chemical and toxicological characterization, hazard identification and mitigation.

Journal of hazardous materials·2026
Same author

3D-Printed Composites Filled with Carbon Nanotubes and Barium Titanate for Electromagnetic Applications.

Polymers·2026
Same author

World Ocean Database 2023: A Foundational Data Resource for and by the Global Ocean and Coastal Communities.

Scientific data·2026
Same author

Unraveling Quinone Degradation Enables Stabilization Using Redox Helpers in Biological and Electrochemical Systems.

Journal of the American Chemical Society·2026
Same journal

Fractionation-Free Protein Corona Quantification Through Synchrotron-Based Small-Angle X-ray Scattering.

Small methods·2026
Same journal

Coronamicroparticle Arrays with Stable Superamphiphobicity.

Small methods·2026
Same journal

Spatial Tail Design in Ionizable Lipids Enhances the Safety and Efficacy of mRNA Delivery.

Small methods·2026
Same journal

Transforming Paper into Plasmonic Sensors: One-Step Fabrication of High-Enhancement SERS Nanosubstrates via Surface Energy Control.

Small methods·2026
Same journal

Analytical Ultracentrifugation in Different High-Density Media Allows to Assess Heterogeneity of mRNA-Lipid Nanoparticles.

Small methods·2026
Same journal

Single Metal Atoms for Energy Storage and Conversion: Recent Advances, Challenges, and Future Perspectives.

Small methods·2026
See all related articles

Related Experiment Video

Updated: Jan 11, 2026

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.8K

Versatile High-Sensitivity EPR Using Superconducting Spiral Microresonators.

Gediminas Usevičius1, Mantas Šimėnas1, Blaise L Geoghegan2

  • 1Faculty of Physics, Vilnius University, Sauletekio 3, LT-10257, Vilnius, Lithuania.

Small Methods
|November 13, 2025
PubMed
Summary
This summary is machine-generated.

Researchers enhanced electron paramagnetic resonance (EPR) spectroscopy sensitivity by over 1000 times using superconducting microresonators. This breakthrough improves the study of paramagnetic centers in various scientific fields.

Keywords:
DEEREPR (Electron paramagnetic resonance)microresonatorsensitivity

More Related Videos

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

6.0K
Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
09:40

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

22.7K

Related Experiment Videos

Last Updated: Jan 11, 2026

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.8K
High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

6.0K
Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
09:40

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

22.7K

Area of Science:

  • Physics
  • Chemistry
  • Materials Science

Background:

  • Electron paramagnetic resonance (EPR) spectroscopy is vital for studying paramagnetic centers across biology, chemistry, and quantum technologies.
  • Conventional EPR instrumentation, while established, has limitations in sensitivity for certain applications.

Purpose of the Study:

  • To significantly increase the spin number sensitivity of X-band pulsed EPR.
  • To achieve this enhancement while maintaining compatibility with existing EPR instruments and sample conditions.

Main Methods:

  • Fabrication of planar spiral-shaped microresonators using yttrium barium copper oxide (YBCO) high-temperature superconductor.
  • Integration of a 7 nL mode volume microresonator into a standard EPR tube for use in conventional EPR cavities.
  • Utilization of microfluidic microstructures matched to the microresonator's mode profile for sample placement.

Main Results:

  • Achieved a three-order-of-magnitude increase in spin number sensitivity.
  • Demonstrated high-fidelity spin control with a sensitivity of 10^7 spins/G/√Hz.
  • Validated performance through pulsed EPR experiments including dipolar and hyperfine spectroscopies on standard samples.

Conclusions:

  • Superconducting microresonators offer a versatile and readily applicable tool for dramatically enhancing EPR sensitivity.
  • This advancement broadens the scope and applicability of EPR spectroscopy in diverse scientific disciplines.
  • The developed approach paves the way for more sensitive investigations of paramagnetic species.