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Updated: Jan 6, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
Proteomic Identification of Microproteins Translated from Long Non-coding RNA
Jing Zhang1, Zi-Hong Fan1, Qing-Yu He2
1MOE Key Laboratory of Tumor Molecular Biology, College of Life Science and Technology, Jinan University, Guangzhou, China.
Abstract:
The identification and exploration of microproteins encoded by long noncoding RNA (lncRNA) genes have the potential to revolutionize our understanding of human biology and expedite medical breakthroughs. Proteomics enables the identification and quantification of thousands of proteins present in cells, tissues or organisms, with LC-MS/MS serving as a pivotal technique in current proteomic research. Here, we describe a comprehensive suite of integrated methods to enhance proteomic analysis for the identification of lncRNA-encoded microproteins. This approach combines tricine gel electrophoresis, in-gel digestion, the establishment of a customized microprotein reference database, and LC-MS/MS analysis.
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