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Updated: Jan 6, 2026

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
Published on: January 13, 2023
Using Untargeted Mass Spectrometry-Based Proteomics to Identify Putative Microprotein Biomarkers from Patient Tampons
Gordon T Luu1, Conrad Fihn2, Laura M Sanchez2
1Bruker Scientific, LLC, Billerica, MA, USA. gordon.luu@bruker.com.
Abstract:
Mass spectrometry-based proteomics is a versatile technique that facilitates the study of microproteins as biomarkers and potential translational applications by utilizing liquid chromatography-trapped ion mobility-tandem mass spectrometry (LC-TIMS-MS/MS) and matrix assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Importantly, MS has been shown to be compatible with complex biological samples (i.e., mammalian biofluids and patient samples). Here we describe a workflow combining: (1) top-down proteomics for analysis of intact microproteins via MALDI-TOF MS and (2) bottom-up proteomics for analysis of enzymatically digested microproteins via LC-TIMS-MS/MS to annotate putative microproteins of interest from a complex biological sample: patient-derived tampons.
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