Related Experiment Video
Updated: Jan 11, 2026
07:25
Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans
Published on: December 9, 2022
2.0K
AMPK opens the door to organelle memory and longevity
1Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA.
Trends in Cell Biology
|November 16, 2025
Summary
AMP-activated protein kinase (AMPK) stabilizes nucleoporin NUP50, activating gene expression for lipid breakdown and longevity. This reveals the nuclear pore complex (NPC) as a key energy-sensing hub.
Area of Science:
- Cellular biology
- Molecular mechanisms of metabolism
- Gene regulation
Background:
- Nutrient sensors are crucial for cellular energy status.
- Metabolic information is relayed to reprogram gene expression.
- The nuclear pore complex (NPC) role in gene regulation is under investigation.
Purpose of the Study:
- To identify novel signaling pathways linking cellular energy status to gene expression.
- To elucidate the role of AMP-activated protein kinase (AMPK) in metabolic control.
- To investigate the function of nucleoporin 50 (NUP50) in transcriptional regulation.
Main Methods:
- Biochemical assays to study protein-protein interactions.
- Gene expression analysis to identify transcriptional targets.
- Cellular models to assess metabolic phenotypes and longevity.
Main Results:
- An AMP-activated protein kinase (AMPK)-NUP50 signaling axis was identified.
- AMPK directly stabilizes nucleoporin NUP50.
- Stabilization of NUP50 activates transcriptional programs promoting lipid catabolism and longevity.
Conclusions:
- The nuclear pore complex (NPC) functions as a dynamic effector.
- NPCs couple cellular energy sensing to transcriptional control.
- The AMPK-NUP50 axis represents a novel mechanism for metabolic homeostasis and longevity.
More Related Videos
08:15Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs
Published on: August 15, 2025
975
10:39A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
Published on: September 17, 2020
6.7K
Related Concept Videos
01:37Mitochondria
19.5K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
19.5K
01:22PI3K/mTOR/AKT Signaling Pathway
5.3K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
5.3K
01:25cAMP-dependent Protein Kinase Pathways
8.3K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
8.3K
01:27Autophagy
5.6K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.6K
01:23Role of Neurotransmitters in Memory
2.4K
Neurotransmitters are integral to the brain's communication system, enabling neurons to transmit signals across synapses. This chemical exchange underpins various cognitive functions, including memory processes. The role of neurotransmitters in memory is multifaceted, influencing the encoding, consolidation, and retrieval of memories through their action on different neural circuits.
Glutamate and Synaptic Plasticity
Glutamate, the brain's main excitatory neurotransmitter, is...
Glutamate and Synaptic Plasticity
Glutamate, the brain's main excitatory neurotransmitter, is...
2.4K
01:07MAPK Signaling Cascades
7.8K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.8K