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Updated: Jan 11, 2026

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
Published on: October 23, 2018
Rutin inhibits hepatic gluconeogenesis and increases glycogen synthesis through the IRS/PI3K/akt signaling pathway in
Qiu-Hong Li1, Ming-Xing Lu1, Yu-Han Feng1
1School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, China.
Abstract:
Rutin, a dietary flavonoid, can relieve insulin resistance to improve hyperglycemia, while the precise mechanism remains unclear. In this study, we found that rutin bound well to the insulin receptor, alleviated glucosamine-induced insulin resistance in HepG2 cells and observably increased glucose consumption and glucose uptake in vitro. Furthermore, rutin increased the levels of IRS-1, IRS-2, PI3K, p-AKT, p-GSK3β and p-FOXO1 and decreased the expression of p-IRS-1, p-GS, PEPCK and G6Pase, indicating that rutin could promote glycogen synthesis and inhibit gluconeogenesis via the IRS/PI3K/Akt signaling pathway. Overall, the findings confirmed that rutin potentially mitigates glucosamine-induced insulin resistance in hepatocytes via activation of IRS/PI3K/Akt pathways.
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