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Updated: Jun 18, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Engineering Two-Dimensional Nanobody-Origami Architectures for Enhanced Antiviral Activity.

Tingjie Song^1,2,3, Jazmin Galván Achi⁴, Varada Anirudhan⁴

¹Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

|November 17, 2025

Summary

This summary is machine-generated.

Researchers developed a DNA origami platform to precisely arrange nanobodies (Nb) for potent antiviral therapies. This nanoscale engineering significantly boosted viral binding and neutralization against SARS-CoV-2 and HIV-1.

Keywords:

Designer DNA nanoarchitecture Multivalency Nanobody Pattern matching Viral inhibitor

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Engineering Antiviral Agents via Surface Plasmon Resonance

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Published on: June 14, 2022

Area of Science:

Nanotechnology
Virology
Immunology

Background:

Nanoscale organization of binding ligands is a key strategy for combating viral infections.
Multivalent nanobodies (Nb) show promise as antiviral agents.
Controlling the spatial arrangement of Nbs is crucial for enhancing their efficacy.

Purpose of the Study:

To develop a programmable 2D DNA origami platform for nanoscale control of multivalent Nb spatial patterns.
To engineer origami-Nb nanoarchitectures with rationally designed Nb patterns for broad-spectrum antiviral applications.
To evaluate the impact of Nb spatial patterns on viral binding affinity and neutralization potency.

Main Methods:

Utilized a 2D DNA origami platform for programmable spatial positioning of Nbs.
Employed site-selective Nb-DNA conjugation for precise Nb attachment.
Synthesized origami-Nb nanoarchitectures with Nb patterns designed to mimic viral surface protein geometry.
Tested the efficacy of engineered nanoarchitectures against SARS-CoV-2 and HIV-1.

Main Results:

Achieved nanoscale control over Nb spatial patterns on a 2D DNA origami platform.
Demonstrated significantly enhanced viral binding affinity and neutralization potency with optimized Nb configurations.
For SARS-CoV-2, a triangular Nb pattern achieved an IC50 of 1.52 nM, a 171-fold improvement over monomeric Nbs.
For HIV-1, an optimal Nb pattern increased neutralization efficiency by 233-fold.

Conclusions:

The programmable 2D DNA origami platform enables precise nanoscale control of Nb spatial patterns.
Spatially optimized Nb patterns lead to significantly enhanced viral binding and neutralization.
This generalizable strategy offers a promising approach for engineering potent antiviral inhibitors targeting viral surface antigens.