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Updated: Jan 11, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

Spatial organization of phosphoinositide signaling

Siyu Lai^1,2, Wenbo Huang^1,2, Kangmin He^1,2

¹Laboratory of Integrative Physiology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

|November 18, 2025

Summary

Mammalian cells utilize seven phosphoinositide species, whose precise spatial organization is crucial for cell function and signaling. Dysregulation of this signaling is linked to human diseases.

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Mammalian cells express seven distinct phosphoinositide species.
Advanced tools have significantly improved understanding of phosphoinositide spatial distribution, functions, and regulation.
Phosphoinositides are tightly regulated by lipid kinases, phosphatases, and lipid transfer proteins.

Purpose of the Study:

To provide an overview of current insights into the spatial organization of phosphoinositide signaling.
To highlight the key roles of phosphoinositide spatial organization in regulating membrane dynamics and signal transduction.
To discuss the involvement of phosphoinositide signaling in various cellular compartments and its link to human diseases.

Main Methods:

This review synthesizes current knowledge from various research studies.
It focuses on insights gained through advanced labeling, imaging, and manipulation tools.
The review examines phosphoinositide distribution and function across multiple cellular organelles.

Main Results:

Each phosphoinositide exhibits a unique and dynamic spatial distribution at subcellular and suborganelle levels.
This spatial organization is critical for membrane trafficking, organelle identity, signal transduction, and other cellular processes.
Dysregulation of spatial phosphoinositide signaling is implicated in various human diseases.

Conclusions:

The spatial organization of phosphoinositides is fundamental to cellular function and signaling.
Understanding these spatial dynamics is key to comprehending cellular processes and disease mechanisms.
Further research into phosphoinositide spatial signaling holds therapeutic potential for human diseases.

Keywords:

endosome nucleus phosphoinositide plasma membrane signaling spatial organization

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