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Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

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Published on: January 5, 2024

ISAba1-mediated bla_ADC disruption restores third-generation cephalosporin susceptibility in a clinical Acinetobacter

Carolina S Nodari^1,2, Qinqin Bai^2,3, Rodrigo Cayô^1,4,5

¹Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil.

Antimicrobial Agents and Chemotherapy

|November 18, 2025

Abstract:

This study describes the effects of the insertion of ISAba1 in the intrinsic bla_ADC gene of an Acinetobacter baumannii isolate showing co-resistance to carbapenems and colistin. Isolate 52971 showed low ceftazidime MIC values due to the disruption of bla_ADC-195 by this insertion sequence. The introduction of an intact bla_ADC in isolate 52971 increased ceftazidime MIC. The potential of insertion sequences to inactivate chromosome-encoded resistance determinants should be explored.

Keywords:

Acinetobacter-derived cephalosporinase carbapenem resistance colistin resistance insertion sequences

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