Bioinspired electrochemical supercapacitor: peptide-driven osteosarcoma detection via hydroxyapatite formation and...

Osteosarcoma, a prevalent age-related condition, often goes undiagnosed due to expensive and invasive detection methods. This study presents a novel, cost-effective, non-invasive electrochemical sensor for Osteosarcoma detection, leveraging peptide probes to selectively recognize key biomarkers like iron ions and osteocalcin. Using a phospholipid monolayer and a conductive substrate, the sensor utilizes peptide probes containing a tripeptide iron-binding sequence and an osteocalcin sequence to detect ferroptosis-induced iron ions and elevated osteocalcin levels, both indicative of early-stage Osteosarcoma. Electrochemical modulation facilitates the covalent assembly of osteocalcin into nanoscale aggregates, significantly amplifying the sensor's signal. This design avoids the need for complex antibodies or nanomaterials, enhancing affordability and simplicity. By integrating everyday components like toothpaste to form a low baseline signal and utilizing saliva as the sample, the sensor offers high sensitivity and a low-cost alternative to traditional diagnostic methods. This innovative approach combines bioinspired materials and electrochemical techniques to provide a promising solution for early Osteosarcoma detection, addressing the pressing need for accessible diagnostics in aging populations.