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Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
Published on: February 6, 2021
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Epithelial-derived basement membranes help orchestrate crypt-villus patterning in vitro
Janny Pineiro-Llanes1, Mashael F Aldossari2, Rodrigo Cristofoletti1
1Department of Pharmaceutics, College of Pharmacy, University of Florida, Orlando, FL, USA.
Developmental Cell
|November 18, 2025
Abstract:
Intestinal organoid culture has widely depended on exogenous laminin-rich matrices to provide a supportive environment for organoid development. In this issue of Developmental Cell, Chrisnandy and Lütolf now report that human and mouse intestinal epithelial cells can secrete their own basement membrane, enabling organoid formation even without exogenous laminin.

