Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flexible artificial compound eye cameras for ultrawide continuous tracking in mixed reality.

Nature communications·2026
Same author

Cr(VI)-Responsive Ink with Four-Dimensional Printing of an Ultracompact Hydrogel Optical Fiber Microsensor.

ACS sensors·2025
Same author

Ultrafast Laser 3D Nanolithography of Fiber-Integrated Silica Microdevices.

Nano letters·2024
Same author

An Optical Fiber-Based Nanomotion Sensor for Rapid Antibiotic and Antifungal Susceptibility Tests.

Nano letters·2024
Same author

Super-Resolution Second-Harmonic Generation Imaging with Multifocal Structured Illumination Microscopy.

Nano letters·2023
Same author

Fiber-integrated cantilever-based nanomechanical biosensors as a tool for rapid antibiotic susceptibility testing.

Biomedical optics express·2023

Related Experiment Video

Updated: Jan 11, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

7.8K

Fiber-Tip Shear Force Probe for Single-Cell Adhesion Force Measurements.

Mengqiang Zou1, Yanping Chen1, Yu Liu1

  • 1Chongqing Key Laboratory of Autonomous Navigation and Microsystems, Chongqing Engineering Research Center of Intelligent Sensing Technology and Microsystem, School of Electronic Science and Engineering, Chongqing University of Posts and Telecommunications, Chongqing 400065, China.

ACS Sensors
|November 19, 2025
PubMed
Summary

A novel fiber-tip shear force probe (FSFP) enables label-free, accurate single-cell adhesion force measurement. This flexible, fast microforce sensor offers new possibilities for cell biology research.

Keywords:
biosensorcell adhesionoptical fiber sensorsingle-cell analysistwo-photon polymerization

More Related Videos

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

10.8K
Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions
06:37

Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions

Published on: June 16, 2018

6.0K

Related Experiment Videos

Last Updated: Jan 11, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

7.8K
Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

10.8K
Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions
06:37

Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions

Published on: June 16, 2018

6.0K

Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Single-cell adhesion is vital in cell biology.
  • Existing methods like AFM have limitations (complexity, labeling).
  • Accurate measurement under physiological conditions is needed.

Purpose of the Study:

  • To develop a miniature fiber-tip shear force probe (FSFP) for precise single-cell adhesion force measurement.
  • To overcome limitations of current cell adhesion measurement techniques.
  • To enable label-free and in-situ analysis of cell adhesion dynamics.

Main Methods:

  • Designed and fabricated a shear force probe integrated onto a single-mode fiber tip using femtosecond laser two-photon polymerization.
  • Established the relationship between FSFP spectral output and applied force.
  • Measured single-cell adhesion forces of MCF-7 breast cancer cells on different substrates.

Main Results:

  • Achieved high microforce sensitivity (2.81 nm/μN) and a minimal detectable force of 7.1 nN.
  • Demonstrated greater cell adhesion on stiffer glass substrates compared to polymer substrates.
  • Observed a >2-fold decrease in adhesion force after trypsin stimulation.
  • Correlated cell morphology (shuttle shape) and actin distribution with substrate stiffness.

Conclusions:

  • The miniature FSFP provides a flexible, fast, and label-free method for accurate single-cell adhesion force measurement.
  • This technology opens new avenues for single-cell analysis under physiological conditions.
  • Findings highlight the influence of substrate stiffness and enzymatic treatment on cell adhesion.