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Updated: Jan 11, 2026
Engineering a Bilayered Hydrogel to Control ASC Differentiation
Published on: May 25, 2012
Anti-infection and healing promotion of skin ECM hydrogel
1School of Nursing, Jilin Engineering Vocational College, Siping, China.
Objective:
Diabetic wounds are slow to heal, causing pain and increasing medical burden. This study aims to develop a skin extracellular matrix (ECM) hydrogel containing ciprofloxacin (CIP) and S-nitrosoglutathione (GSNO) to evaluate its antimicrobial and wound healing effects.
Methods:
Porcine skin was used to extract ECM hydrogel via decellularization, followed by loading with CIP and GSNO. Scanning Electron Microscopy (SEM) was used for microstructure and drug distribution characterization. In vitro antibacterial tests, along with human umbilical vein endothelial cell (HUVEC) proliferation and migration assays, were conducted. Animal studies on diabetic mice and histological analysis were performed.
Results:
The ECM hydrogel with CIP and GSNO demonstrated uniform drug distribution and significant antibacterial effects, with a 99.2% antibacterial rate against Staphylococcus aureus. In vitro, the hydrogel enhanced HUVEC cell proliferation by 35% and migration by 51%. In diabetic mice, wound healing was accelerated, with a 95.2% healing rate at 14 days, 56.1% higher than the control group. Additionally, bacterial load was reduced by 77.6%, and angiogenesis and collagen fiber formation were promoted.
Conclusion:
This study successfully developed a skin ECM hydrogel with dual antimicrobial and healing effects, offering a promising strategy for diabetic wound treatment.
