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Related Experiment Videos

Thymus-derived rosettes are not "helper" cells.

B E Elliott, J S Haskill, M A Axelrad

    The Journal of Experimental Medicine
    |November 1, 1973
    PubMed
    Summary
    This summary is machine-generated.

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    T rosettes do not function as helper cells in the direct plaque-forming cell (PFC) response. B-rosette fractions contain PFC precursors, while nonrosette lymphocytes act as helper cells in this immune response.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Identifying distinct lymphocyte populations is crucial for understanding immune responses.
    • Sheep red blood cells (SRBC) are commonly used antigens in immunological studies.
    • T rosettes and B rosettes represent specific cellular interactions in the spleen.

    Purpose of the Study:

    • To determine the functional role of T rosettes, B rosettes, and nonrosette lymphocytes in the direct plaque-forming cell (PFC) response.
    • To investigate the sedimentation properties of PFC precursors and helper cells.

    Main Methods:

    • Normal spleen cells were used to form rosettes against SRBC.
    • T rosettes were stabilized using sodium azide.
    • Velocity sedimentation at unit gravity separated nonrosettes, T rosettes, and B rosettes.

    Related Experiment Videos

  • Fractions were injected into irradiated hosts with bone marrow or thymus cells and antigen.
  • Direct PFC assays were performed 7 days post-injection.

    • Main Results:

    • Synergism with thymus cells was observed exclusively in the B-rosette fraction, indicating PFC precursors sediment as B rosettes.
    • Synergism with bone marrow cells occurred only in the nonrosette small lymphocyte fraction.
    • Helper cells did not bind detectable numbers of SRBC.

    Conclusions:

    • PFC precursors are associated with B rosettes.
    • Helper cells involved in the direct PFC response are found in the nonrosette small lymphocyte fraction.
    • T rosettes are not helper cells in the direct PFC response of bone marrow B cells to SRBC.