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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Comparative Proteomic Profiling of Adrenocortical Neoplasia Using Mass Spectrometry.

Jean Lucas Kremer1, Henrique Sanchez Ortega1, Talita Siqueira-Souza2

  • 1Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo, Av. Prof. Lineu Prestes, 2415, Butantan, São Paulo 05508-000, Brazil.

Journal of Proteome Research
|November 21, 2025
PubMed
Summary

This study compared protein profiles in normal adrenal tissue and various adrenal tumors, identifying key protein differences. These findings offer insights into adrenocortical disease mechanisms and potential therapeutic targets.

Keywords:
adrenal glandadrenocortical neoplasiabioinformaticsproteome

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Area of Science:

  • Endocrinology
  • Proteomics
  • Molecular Biology

Background:

  • Adrenocortical diseases involve complex molecular mechanisms.
  • High-throughput molecular analysis advances understanding of these diseases.

Purpose of the Study:

  • To identify protein signature differences in normal adrenal tissue and various adrenocortical tumors.
  • To gain insights into disease-specific pathogenesis of adrenocortical diseases.
  • To discover potential biomarkers and therapeutic targets.

Main Methods:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for proteomic profiling.
  • Bioinformatic analysis to compare protein expression across normal human adrenal (NHA) tissue, adrenocortical adenomas (ACA), adrenocortical carcinomas (ACC), and primary macronodular adrenocortical hyperplasia (PMAH) tumors.
  • Validation using an independent ACC cohort.

Main Results:

  • Identified 7350 proteins, quantifying 3976 across all samples.
  • Found significant differentially expressed proteins (DEPs) between NHA and tumor types (ACA, ACC, PMAH).
  • Characterized protein differences in ACC vs. ACA and in PMAH with and without ARMC5 mutations, with validation confirming findings.

Conclusions:

  • Comprehensive proteomic profiling provides insights into normal adrenal function and tumor-associated changes.
  • The study highlights potential protein biomarkers and therapeutic targets for adrenocortical diseases.
  • This research significantly contributes to understanding the pathogenesis of adrenocortical diseases.