Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K
RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of microprotein-coding intronic polyadenylation isoforms and function in genotoxic anticancer drug response.

Genome biology·2025
Same author

Hexokinase 2 is an RNA-binding protein that regulates mRNA translation independently of glycolysis and induces melanoma cell proliferation.

PLoS biology·2025
Same author

The unconventional RNA-binding activity of metabolic enzymes.

Biochimie·2025
Same author

Author Correction: Genotoxic stress triggers Scd6-dependent regulation of translation to modulate the DNA damage response.

EMBO reports·2025
Same author

Genotoxic stress triggers Scd6-dependent regulation of translation to modulate the DNA damage response.

EMBO reports·2025
Same author

m<sup>6</sup>A RNA methylation controls salivary gland epithelial cell function and has a protective role in Sjögren's disease.

Annals of the rheumatic diseases·2024

Related Experiment Video

Updated: Jan 6, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
10:00

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

28.8K

Polysome Profiling: A Method for Global and Targeted Analysis of mRNA Translation.

Lucilla Fabbri1,2, Ana Luisa Dian3,4, Céline M Labbé3,4

  • 1Institut Curie, PSL Research University, CNRS UMR 3348, INSERM U1278, Orsay, France. lucilla.fabbri@curie.fr.

Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2025
PubMed
Summary
This summary is machine-generated.

This study details polysome profiling, a method to analyze messenger RNA (mRNA) translation. Understanding mRNA translation is crucial for immune cell function and overall health.

Keywords:
Polysome profilingPost-transcriptional gene regulationmRNA translation

More Related Videos

An Easy Method for Plant Polysome Profiling
11:09

An Easy Method for Plant Polysome Profiling

Published on: August 28, 2016

13.2K
Polysome Profiling without Gradient Makers or Fractionation Systems
05:56

Polysome Profiling without Gradient Makers or Fractionation Systems

Published on: June 1, 2021

6.1K

Related Experiment Videos

Last Updated: Jan 6, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
10:00

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

28.8K
An Easy Method for Plant Polysome Profiling
11:09

An Easy Method for Plant Polysome Profiling

Published on: August 28, 2016

13.2K
Polysome Profiling without Gradient Makers or Fractionation Systems
05:56

Polysome Profiling without Gradient Makers or Fractionation Systems

Published on: June 1, 2021

6.1K

Area of Science:

  • Molecular Biology
  • Immunology
  • Gene Expression Regulation

Background:

  • Messenger RNA (mRNA) translation is a key regulatory mechanism controlling cellular functional output.
  • Tightly regulated mRNA translation is vital for immune cell homeostasis and function.
  • Analyzing translational status offers insights into physiological and pathological conditions.

Purpose of the Study:

  • To present a standardized protocol for polysome profiling.
  • To enable the analysis of mRNA translation at both individual transcript and genome-wide scales.
  • To facilitate research into the regulatory mechanisms of mRNA translation.

Main Methods:

  • Polysome profiling protocol.
  • Analysis of mRNA translation.
  • Genome-wide and individual transcript analysis.

Main Results:

  • A routinely used protocol for polysome profiling is described.
  • The method allows for comprehensive analysis of mRNA translation.
  • The protocol is applicable to both individual transcripts and genome-wide studies.

Conclusions:

  • Polysome profiling is an effective method for studying mRNA translation.
  • This protocol aids in understanding gene expression regulation in various cellular contexts, particularly immune cells.
  • The described method provides valuable insights into factors influencing cellular phenotypes.