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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
Published on: October 28, 2014
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Polysome Profiling: A Method for Global and Targeted Analysis of mRNA Translation
Lucilla Fabbri1,2, Ana Luisa Dian3,4, Céline M Labbé3,4
1Institut Curie, PSL Research University, CNRS UMR 3348, INSERM U1278, Orsay, France. lucilla.fabbri@curie.fr.
Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2025
Summary
This study details polysome profiling, a method to analyze messenger RNA (mRNA) translation. Understanding mRNA translation is crucial for immune cell function and overall health.
Area of Science:
- Molecular Biology
- Immunology
- Gene Expression Regulation
Background:
- Messenger RNA (mRNA) translation is a key regulatory mechanism controlling cellular functional output.
- Tightly regulated mRNA translation is vital for immune cell homeostasis and function.
- Analyzing translational status offers insights into physiological and pathological conditions.
Purpose of the Study:
- To present a standardized protocol for polysome profiling.
- To enable the analysis of mRNA translation at both individual transcript and genome-wide scales.
- To facilitate research into the regulatory mechanisms of mRNA translation.
Main Methods:
- Polysome profiling protocol.
- Analysis of mRNA translation.
- Genome-wide and individual transcript analysis.
Main Results:
- A routinely used protocol for polysome profiling is described.
- The method allows for comprehensive analysis of mRNA translation.
- The protocol is applicable to both individual transcripts and genome-wide studies.
Conclusions:
- Polysome profiling is an effective method for studying mRNA translation.
- This protocol aids in understanding gene expression regulation in various cellular contexts, particularly immune cells.
- The described method provides valuable insights into factors influencing cellular phenotypes.
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