Protocol to establish rice root aphid colonies and experiments in greenhouse settings

The rice root aphid (RRA) Rhopalosiphum rufiabdominalis (Sasaki) is a cosmopolitan species that attacks the root system of many plants grown in fields and greenhouses, including hydroponic production systems. Here, we present a protocol to establish consistent RRA colonies used to infest plants and to set up experiments in greenhouse settings. We describe steps for researching and understanding RRA colonies and establishing and maintaining the colony. We then provide suggestions for conducting studies in a greenhouse setting.