Jove
Visualize
Contact Us

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

8.4K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromosome architecture affects virulence and competitiveness in <i>Agrobacterium tumefaciens</i> C58.

Science advances·2025
Same author

Enhancing <i>Agrobacterium</i>-mediated plant transformation efficiency through improved ternary vector systems and auxotrophic strains.

Frontiers in plant science·2024
Same author

Immune cell infiltration and drug response in glioblastoma multiforme: insights from oxidative stress-related genes.

Cancer cell international·2024
Same author

Periodontitis and osteoporosis: a two-sample Mendelian randomization analysis.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2024
Same author

Diagnostic Performance of Angiography-Derived Quantitative Flow Ratio in Complex Coronary Lesions.

Circulation. Cardiovascular imaging·2024
Same author

Morusin Alleviates Aortic Valve Calcification by Inhibiting Valve Interstitial Cell Senescence Through Ccnd1/Trim25/Nrf2 Axis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 10, 2026

Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens
08:00

Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens

Published on: October 27, 2023

4.7K

IMAGE: INTEGRATE-Mediated Agrobacterium Genome Engineering.

Ephraim Aliu1,2,3, Liang-Chun Chen1,2,3, Keunsub Lee1,2

  • 1Department of Agronomy, Iowa State University, Ames, IA, United States.

Frontiers in Microbiology
|November 24, 2025
PubMed
Summary

Researchers optimized the CRISPR RNA-guided transposase system, INTEGRATE, for precise Agrobacterium genome engineering. This standardized protocol facilitates Agrobacterium strain development for plant biotechnology applications.

Keywords:
Agrobacterium rhizogenesAgrobacterium spp.Agrobacterium tumefaciensCRISPR integraseCre-loxdisarminglarge DNA fragment deletiontargeted DNA insertion

More Related Videos

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K
Site-specific Bacterial Chromosome Engineering: &#934;C31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.1K

Related Experiment Videos

Last Updated: Jan 10, 2026

Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens
08:00

Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens

Published on: October 27, 2023

4.7K
Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K
Site-specific Bacterial Chromosome Engineering: &#934;C31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.1K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Precise engineering of Agrobacterium strains is vital for advancing plant biotechnology.
  • The CRISPR RNA-guided transposase system, INTEGRATE, offers an efficient method for genetic modification in Agrobacterium.
  • Current applications of INTEGRATE in Agrobacterium strain engineering require further exploration and standardization.

Purpose of the Study:

  • To present a standardized and optimized workflow for utilizing the INTEGRATE system in Agrobacterium.
  • To address and overcome common challenges associated with INTEGRATE implementation, including crRNA design, transformation efficiency, and vector eviction.
  • To expand the genetic engineering toolkit for Agrobacterium to support functional genomics and strain development.

Main Methods:

  • Development of a standardized protocol for CRISPR-RNA guided transposase (INTEGRATE) system application in Agrobacterium.
  • Optimization of target site selection, crRNA spacer cloning, and Agrobacterium transformation procedures.
  • Implementation of screening methods for targeted insertion, Cre/loxP-mediated deletion, and vector removal.

Main Results:

  • A robust workflow for targeted genome modifications in Agrobacterium using the INTEGRATE system was established.
  • The protocol successfully demonstrated the domestication of Agrobacterium rhizogenes K599 by deleting the T-DNA region and creating auxotrophy.
  • The developed methods enhance efficiency and reproducibility of Agrobacterium genome engineering.

Conclusions:

  • The optimized INTEGRATE protocol provides a valuable resource for researchers performing Agrobacterium genome engineering.
  • This standardized workflow facilitates the development of improved Agrobacterium strains for plant transformation applications.
  • The study promotes broader adoption of advanced genetic engineering tools in Agrobacterium, driving innovation in plant biotechnology.