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Related Concept Videos

Kidney Transplant I: Introduction01:28

Kidney Transplant I: Introduction

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A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
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Kidney Transplant II: Surgical Procedure01:26

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Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living...
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Related Experiment Video

Updated: Jan 10, 2026

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
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Cellular Responses During Kidney Normothermic Machine Perfusion Reflect Graft Outcomes.

Shengbing Li1, Hector Tejeda-Mora1, Julia S Slagter2

  • 1Division of Nephrology and Transplantation, Department of Internal Medicine, Erasmus MC Transplant Institute, University Medical Center Rotterdam, The Netherlands.

Kidney International Reports
|November 24, 2025
PubMed
Summary
This summary is machine-generated.

Normothermic machine perfusion (NMP) alters kidney gene expression, potentially indicating higher early rejection risk in certain donor kidneys. This cellular response analysis helps predict transplant outcomes.

Keywords:
acute rejectionkidney transplantationnormothermic machine perfusionquantitative real-time PCRsingle nucleus RNA sequencing

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Last Updated: Jan 10, 2026

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Area of Science:

  • Nephrology
  • Transplantation immunology
  • Genomics

Background:

  • Donor kidneys, especially from older donors or after circulatory death, are vulnerable to injury and immune attack post-transplant.
  • Current methods lack reliable tools to predict the outcome of donor kidney transplantation.

Purpose of the Study:

  • To investigate if cellular responses during normothermic machine perfusion (NMP) correlate with post-transplant outcomes in donor kidneys.
  • To identify potential biomarkers for predicting kidney transplant success.

Main Methods:

  • Single nucleus RNA sequencing (snRNA-seq) and quantitative real-time PCR (qPCR) were used on kidney biopsies before and after 2-hour NMP.
  • Post-transplant function was assessed using estimated glomerular filtration rates (eGFRs).

Main Results:

  • NMP significantly altered gene expression in multiple kidney cell types, including upregulation of genes related to ATP production, heat shock proteins (HSPs), and protein folding.
  • No significant gene expression differences were found between kidneys with delayed graft function (DGF) and non-DGF kidneys.
  • Elevated HSP expression post-NMP was observed in kidneys experiencing acute rejection within 10 days of transplantation.

Conclusions:

  • Normothermic machine perfusion (NMP) influences kidney cell gene expression profiles.
  • Differential gene expression patterns following NMP may indicate an increased risk for early kidney transplant rejection.