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Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
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Multi-immersion Oblique Plane Microscope (miOPM): A reconfigurable platform for high-resolution Light-Sheet

Bingying Chen1,2, Alfred Millett-Sikking3, Seweryn Gałecki1,2,4

  • 1Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX, 75390, U.S.A.

Biorxiv : the Preprint Server for Biology
|November 24, 2025
PubMed

Insights

We developed the multi-immersion oblique plane microscope (miOPM), an adaptable Light-Sheet Fluorescence Microscopy (LSFM) platform. This versatile tool enhances high-resolution 3D imaging across diverse biological samples and scales.

Area of Science:

  • Microscopy
  • Biophysics
  • Optical Engineering

Background:

  • Light-Sheet Fluorescence Microscopy (LSFM) offers gentle, rapid, and efficient volumetric imaging.
  • LSFM variants are often narrowly optimized, limiting broad applicability.
  • A need exists for adaptable LSFM platforms for diverse biological samples.

Purpose of the Study:

  • Introduce the multi-immersion oblique plane microscope (miOPM) as an adaptable LSFM platform.
  • Demonstrate miOPM's capability for high-resolution imaging across various spatial scales.
  • Expand the adaptability and ease of use of LSFM for advanced 3D imaging.

Main Methods:

  • Developed the multi-immersion oblique plane microscope (miOPM).
  • Enabled seamless interchangeability of oil, water, and air objectives.
  • Validated performance across a refractive index range of 1.33 - 1.51.

Main Results:

  • miOPM supports high-resolution imaging from subcellular dynamics to whole organisms and cleared tissues.
  • Achieved diffraction-limited performance across a wide refractive index range.
  • Demonstrated compatibility with standard sample mounting, clearing protocols, and high-throughput 3D imaging.

Conclusions:

  • The miOPM significantly expands the adaptability and ease of use of LSFM.
  • miOPM facilitates high-resolution 3D imaging of diverse biological specimens at multiple scales.
  • This adaptable LSFM platform is poised to democratize advanced three-dimensional imaging.

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