Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A role for the <i>Stentor</i> syntaxin protein in post-wound cell survival.

Molecular biology of the cell·2026
Same author

Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane Protrusions.

Research square·2026
Same author

Mechanism study of HuanglianKushen decoction intervention in DSS-induced ulcerative colitis mice based on integrative omics.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Axially Swept Light-Sheet Microscopy using scattering and fluorescence contrast mechanisms.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same author

Hybrid solid-liquid optics enable scalable, high-resolution light-sheet microscopy across diverse immersion media.

Nature biotechnology·2026
Same author

Machine learning assisted wavefront sensor.

Proceedings of SPIE--the International Society for Optical Engineering·2026

Related Experiment Video

Updated: Jan 10, 2026

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.1K

Multi-immersion Oblique Plane Microscope (miOPM): A reconfigurable platform for high-resolution Light-Sheet

Bingying Chen1,2, Alfred Millett-Sikking3, Seweryn Gałecki1,2,4

  • 1Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX, 75390, U.S.A.

Biorxiv : the Preprint Server for Biology
|November 24, 2025
PubMed
Summary

We developed the multi-immersion oblique plane microscope (miOPM), an adaptable Light-Sheet Fluorescence Microscopy (LSFM) platform. This versatile tool enhances high-resolution 3D imaging across diverse biological samples and scales.

More Related Videos

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.1K
Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes
08:29

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Published on: September 9, 2020

3.5K

Related Experiment Videos

Last Updated: Jan 10, 2026

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.1K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.1K
Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes
08:29

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Published on: September 9, 2020

3.5K

Area of Science:

  • Microscopy
  • Biophysics
  • Optical Engineering

Background:

  • Light-Sheet Fluorescence Microscopy (LSFM) offers gentle, rapid, and efficient volumetric imaging.
  • LSFM variants are often narrowly optimized, limiting broad applicability.
  • A need exists for adaptable LSFM platforms for diverse biological samples.

Purpose of the Study:

  • Introduce the multi-immersion oblique plane microscope (miOPM) as an adaptable LSFM platform.
  • Demonstrate miOPM's capability for high-resolution imaging across various spatial scales.
  • Expand the adaptability and ease of use of LSFM for advanced 3D imaging.

Main Methods:

  • Developed the multi-immersion oblique plane microscope (miOPM).
  • Enabled seamless interchangeability of oil, water, and air objectives.
  • Validated performance across a refractive index range of 1.33 - 1.51.

Main Results:

  • miOPM supports high-resolution imaging from subcellular dynamics to whole organisms and cleared tissues.
  • Achieved diffraction-limited performance across a wide refractive index range.
  • Demonstrated compatibility with standard sample mounting, clearing protocols, and high-throughput 3D imaging.

Conclusions:

  • The miOPM significantly expands the adaptability and ease of use of LSFM.
  • miOPM facilitates high-resolution 3D imaging of diverse biological specimens at multiple scales.
  • This adaptable LSFM platform is poised to democratize advanced three-dimensional imaging.