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Related Concept Videos

Assessing Body Temperature - Temporal Artery01:19

Assessing Body Temperature - Temporal Artery

1.0K
Here is a stepwise guide to assessing the body temperature at the temporal artery using a temporal artery thermometer
Step 1: Perform hand hygiene and don a fresh pair of gloves to prevent cross-infection and ensure patient safety.
Step 2: Explain the procedure to the patient to establish trust. Clear communication establishes trust with the patient, ensures they understand what to expect, promotes cooperation, and enhances comfort during the procedure.  
Step 3: Assess the patient's...
1.0K
Assessing Body Temperature - Axilla01:14

Assessing Body Temperature - Axilla

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Procedural Guide for Assessing Axillary Body Temperature using a Digital Thermometer:
Step 1: Perform hand hygiene and put on clean gloves to maintain infection control and prevent cross-contamination.
Step 2: Prepare the patient by explaining the procedure to ensure understanding and cooperation. Ensure privacy, expose the axilla, and inform the patient that minimal movement is crucial for an accurate reading.
Step 3: Adjust the patient’s clothing to expose only the axilla. It minimizes...
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Assessing Body Temperature - Oral01:14

Assessing Body Temperature - Oral

1.4K
Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
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Equipments Used to Measure Body Temperature01:13

Equipments Used to Measure Body Temperature

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Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
1.7K
Assessing Body Temperature - Tympanic membrane01:14

Assessing Body Temperature - Tympanic membrane

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Assessing tympanic membrane temperature involves using a tympanic membrane thermometer (TMT). Here is a step-by-step guide:
Step 1: Begin by practicing good hand hygiene to prevent the transmission of microorganisms.
Step 2: Turn on the thermometer and wait until the ready sign appears on the screen to ensure accurate measurement.
Step 3: Slide the probe cover in place to prevent cross-contamination.
Step 4: Instruct the patient to tilt their head to the side for comfort and check for cerumen...
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Related Experiment Video

Updated: Jan 10, 2026

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
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Device-Free Nucleic Acid Detection Enabled by Body Temperature-Assisted DEMBA.

Jianying Yi1, Li Wan2, Jia Ding3

  • 1Department of Clinical Laboratory, Tianjin First Central Hospital, Tianjin 300192, China.

ACS Omega
|November 24, 2025
PubMed
Summary

We developed a novel body temperature endonuclease-dependent molecular beacon assay (DEMBA) for rapid nucleic acid detection. This method enables visual, device-free DNA/RNA testing at 37°C using patient body heat.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Diagnostics

Background:

  • Nucleic acid detection methods often require complex equipment and thermal cycling.
  • There is a need for rapid, accessible, and field-deployable diagnostic tools.

Purpose of the Study:

  • To develop a novel nucleic acid detection method utilizing body temperature and endonuclease activity.
  • To establish a foundation for device-free nucleic acid testing at 37°C.

Main Methods:

  • Developed body temperature endonuclease-dependent molecular beacon assay (DEMBA) probes with restriction endonucleases (Bpu 10I, Bpu 1102I).
  • Utilized NUPACK for secondary structure prediction, agarose gel electrophoresis for analysis, and real-time PCR for fluorescence monitoring.
  • Integrated DEMBA with lateral flow assays (LFAs) for visual detection.

Main Results:

  • DEMBA demonstrated significant fluorescence changes in the presence versus absence of target sequences.
  • Successful nucleic acid detection was achieved at 37°C, leveraging patient body temperature.
  • The assay directly hybridizes with RNA, eliminating reverse transcription and reducing assay time.

Conclusions:

  • DEMBA offers a versatile, rapid, and potentially device-free approach for nucleic acid detection.
  • The method's reliance on body temperature enhances accessibility for point-of-care diagnostics.
  • DEMBA provides a foundation for developing advanced, temperature-independent nucleic acid testing technologies.