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Protein Scaffold DNA-Cu2+ Functional Nanostructure Enable Rapid One-Pot Visual Portable Adaptable Aptasensing
Xianghu Zeng1,2, Wanyu Zhao1, Birong Dong1
1Department of Laboratory Medicine, Med+X Center for Manufacturing, National Clinical Research Center for Geriatrics, State Key Laboratory of Respiratory Health and Multimorbidity, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
ACS Sensors
|November 24, 2025
Summary
A novel "I@I@X" strategy uses DNA nanospheres for rapid, versatile biosensing of biomarkers like insulin-like growth factor 1 and cortisol. This method shows clinical potential for precise laboratory medicine diagnostics.
Area of Science:
- Biotechnology
- Nanotechnology
- Biomedical Engineering
Background:
- DNA nanomaterials offer advanced biosensing capabilities.
- Developing efficient and versatile detection methods is crucial for diagnostics.
Purpose of the Study:
- To introduce a one-step, one-pot synthesis strategy for protein scaffold DNA-Cu2+ functional nanospheres.
- To enable versatile detection of various biomarkers using different signal probes and smartphone output.
Main Methods:
- Synthesized protein scaffold DNA-Cu2+ nanospheres via a one-pot reaction at room temperature.
- Integrated aptamers, DNA tetramers, and Cu2+ for biomarker recognition, signal amplification, and transduction.
- Utilized smartphone RGB output for signal detection within 45+X minutes.
Main Results:
- Successfully synthesized multifunctional nanospheres for sensing.
- Demonstrated detection of insulin-like growth factor 1, cortisol, circulating tumor cells, and tumor-derived exosomes.
- Validated clinical practicability in 137 plasma samples with good consistency with clinical indicators.
Conclusions:
- The
- I@I@X
- strategy provides a straightforward and adaptable method for precise laboratory medicine.
- This approach holds promise for transformative applications in biosensing and diagnostics.
