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Updated: Jan 10, 2026

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Diversity-Oriented Enzymatic Modular Synthesis of N-Glycans Bearing LacdiNAc-Related Epitopes
Xuefei Yin1,2, Xiaoying Zhang2, Kan Zhong2
1National Glycoengineering Research Center, NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao 266237, China.
Abstract:
The disaccharide unit GalNAcβ1,4GlcNAc (LacdiNAc or LDN) and its α1,3-fucosylated derivative LDNF and α2,6-sialylated derivative 6'-SLDN are common epitopes of various N-glycans. Due to their structural complexity, the synthesis of a comprehensive library of N-glycans bearing LacdiNAc-related epitopes remains a significant challenge. Herein, we present a highly efficient enzymatic modular assembly strategy for the collective synthesis of 22 biantennary N-glycans containing LacdiNAc, LDNF, or 6'-SLDN epitopes. This strategy features the site-selective enzymatic preparation of two asymmetric N-glycan core structures, followed by diversity-oriented derivatization to provide 16 hard-to-obtain asymmetric biantennary N-glycans bearing LacdiNAc-related epitopes.
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