Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.1K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural basis of fungal β-1,3-glucan synthase inhibition by caspofungin.

Nature·2026
Same author

VitriFlex: An Open-Source, Modular, and Customizable Robotic Platform for Cryo-EM Grid Preparation.

bioRxiv : the preprint server for biology·2026
Same author

Low-GPX4 drives a sustained drug-tolerant persister state in TNBC by a targetable adaptive FSP1 upregulation.

Redox biology·2025
Same author

Structural Basis of Cold and Menthol Sensing by TRPM8.

bioRxiv : the preprint server for biology·2025
Same author

Structural insights into proteolysis-dependent and -independent suppression of the master regulator DELLA by the gibberellin receptor.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Cryo-EM structures of engineered Shiga toxin-based immunogens capable of eliciting neutralizing antibodies with therapeutic potential against hemolytic uremic syndrome.

Protein science : a publication of the Protein Society·2025

Related Experiment Video

Updated: Jan 10, 2026

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

4.9K

Advances in automation for cryo-electron tomography data collection.

Kedar Sharma1, Mario J Borgnia1

  • 1Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, Durham, NC 27709, USA.

Current Opinion in Structural Biology
|November 25, 2025
PubMed
Summary

Advancements in cryo-electron tomography (Cryo-ET) enhance high-resolution imaging of macromolecular complexes within cells. Innovations improve data collection, image quality, and automation for easier, more powerful structural biology.

More Related Videos

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

11.4K
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

5.7K

Related Experiment Videos

Last Updated: Jan 10, 2026

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

4.9K
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

11.4K
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

5.7K

Area of Science:

  • Structural Biology
  • Biophysics
  • Microscopy

Background:

  • Cryo-electron microscopy (Cryo-EM) is vital for macromolecular structure determination.
  • Cryo-electron tomography (Cryo-ET) visualizes structures in cellular contexts.
  • Cryo-ET faces challenges like low signal-to-noise and radiation sensitivity.

Purpose of the Study:

  • To review recent advancements in Cryo-ET.
  • To highlight strategies improving throughput and resolution.
  • To discuss innovations enhancing user experience and accessibility.

Main Methods:

  • Single particle analysis and cryo-electron tomography (Cryo-ET) with subvolume averaging (SVA).
  • Continuous tilt and parallel acquisition strategies.
  • Montage tomography and advanced aperture designs.
  • Machine learning for automated operation and targeting.

Main Results:

  • Improved signal-to-noise ratios and dose efficiency.
  • Increased observable cellular area via montage tomography.
  • Enhanced automation and remote operation capabilities.
  • Progress towards higher resolution and user-friendly Cryo-ET.

Conclusions:

  • Recent innovations are overcoming key Cryo-ET challenges.
  • These advancements facilitate robust, high-resolution imaging in native environments.
  • Cryo-ET is becoming more accessible and powerful for structural biology.