Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.1K
Alternative RNA Splicing02:18

Alternative RNA Splicing

24.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.6K
RNA Splicing01:32

RNA Splicing

60.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.3K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.5K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.5K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Exon Recombination02:32

Exon Recombination

4.1K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sensory nerve-derived signaling coordinates oropharyngeal structural organization that supports suckling and vocalization in neonatal mice.

Nature communications·2026
Same author

Mapping self-associating chromatin hubs identifies Id proteins as key determinants of exhausted CD8<sup>+</sup> T cell fate.

Nature immunology·2026
Same author

Exhausted CD8<sup>+</sup> T cell fate is programmed by dynamic CTCF-mediated enhancer activation and invariant CTCF-imposed barriers.

Nature immunology·2026
Same author

Epigenetic regulation of mesenchymal BMP signaling directs postnatal organ innervation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

RECQ4 arginine methylation suppresses single-stranded DNA gap accumulation at replication forks.

Nucleic acids research·2026
Same author

Use of AI agents to assess preoperative frailty in cancer patients.

npj digital surgery·2026

Related Experiment Video

Updated: Jan 10, 2026

Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
11:10

Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

Published on: July 6, 2022

2.6K

PRMT1-SFPQ regulates intron retention to control matrix gene expression during craniofacial development.

Julia Raulino Lima, Nicha Ungvijanpunya, Qing Chen

    Biorxiv : the Preprint Server for Biology
    |November 26, 2025
    PubMed
    Summary

    Protein arginine methyltransferase 1 (PRMT1) regulates craniofacial development by controlling splicing. PRMT1 and SFPQ pathway modulates matrix gene expression via intron retention-triggered nonsense-mediated decay in cranial neural crest cells.

    More Related Videos

    Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
    07:26

    Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

    Published on: April 1, 2022

    2.4K
    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
    08:54

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

    7.5K

    Related Experiment Videos

    Last Updated: Jan 10, 2026

    Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
    11:10

    Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

    Published on: July 6, 2022

    2.6K
    Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
    07:26

    Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

    Published on: April 1, 2022

    2.4K
    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
    08:54

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

    7.5K

    Area of Science:

    • Developmental Biology
    • Molecular Biology
    • Genetics

    Background:

    • Spliceosomopathies affect craniofacial development, but mechanisms are unclear.
    • Arginine methylation is a key post-translational modification of splicing factors.
    • Protein arginine methyltransferase 1 (PRMT1) is crucial for craniofacial development.

    Purpose of the Study:

    • Investigate PRMT1's role in splicing regulation within cranial neural crest cells (CNCCs).
    • Elucidate the molecular mechanisms underlying PRMT1's impact on craniofacial development.
    • Identify downstream targets and pathways regulated by PRMT1 in CNCCs.

    Main Methods:

    • Analysis of splicing mechanisms in PRMT1-deficient CNCCs.
    • Quantification of intron retention and mRNA abundance.
    • Identification of PRMT1 substrates using proteomics and genetic approaches.
    • Depletion studies of identified substrates in CNCCs.

    Main Results:

    • PRMT1 deficiency in CNCCs increases intron retention in matrix genes, triggering nonsense-mediated decay (NMD) and reducing matrix mRNA.
    • SFPQ identified as a PRMT1 substrate essential for craniofacial development.
    • SFPQ depletion phenocopies PRMT1 deletion, affecting matrix, Wnt signaling, and neuronal gene expression.
    • Gene length identified as a feature of SFPQ-regulated genes.

    Conclusions:

    • The PRMT1-SFPQ pathway regulates matrix gene expression in CNCCs through intron retention-triggered NMD.
    • This pathway is critical for normal craniofacial development.
    • Findings provide molecular insights into spliceosomopathies affecting the craniofacial region.