Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Other Glycolytic Pathways01:24

Other Glycolytic Pathways

788
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
788
What is Glycolysis?00:56

What is Glycolysis?

176.0K
Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
176.0K
Outcomes of Glycolysis01:13

Outcomes of Glycolysis

106.6K
Nearly all the energy used by cells comes from the bonds that make up complex organic compounds. These organic compounds are broken down into simpler molecules, such as glucose. As a result, cells extract energy from glucose over many chemical reactions—a process called cellular respiration.
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...
106.6K
Glycolysis01:23

Glycolysis

1.5K
Glycolysis, the Embden-Meyerhof pathway, is a central metabolic pathway involved in glucose catabolism. It is highly conserved across most organisms, reflecting its fundamental role in cellular energy production. This process occurs in the cytoplasm and can function both in the presence and absence of oxygen, making it versatile for various organisms and environmental conditions.Stages of GlycolysisGlycolysis is a ten-step pathway that converts glucose into pyruvate, generating a net gain of...
1.5K
Energy-requiring Steps of Glycolysis01:20

Energy-requiring Steps of Glycolysis

171.1K
Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
171.1K
Glycolysis: Preparatory Phase01:21

Glycolysis: Preparatory Phase

16.4K
In cellular metabolism (the complete breakdown of glucose to extract energy),  glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
16.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Animal husbandry and environmental conditions are associated with cefotaxime-resistant Escherichia coli in yard soil in peri-urban Malawi.

PLOS global public health·2026
Same author

SynaptoTagMe, a toolkit for in vivo mapping and modulating neurotransmission at single-cell resolution.

eLife·2026
Same author

Clinical Outcomes of 1.5-Stage Arthroplasty for Native Joint Septic Arthritis of the Hip and Knee: A Retrospective Cohort Study.

Arthroplasty today·2026
Same author

Experience-Dependent Gain Modulation Drives Thermosensory Responses in Behavior.

bioRxiv : the preprint server for biology·2026
Same author

Physical contact reveals a hidden layer of cortical architecture.

bioRxiv : the preprint server for biology·2026
Same author

Long-term editing of brain circuits using an engineered electrical synapse.

Nature·2026

Related Experiment Video

Updated: Jan 10, 2026

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
08:40

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

Published on: November 21, 2016

30.7K

Spatial Partitioning of Core Glycolysis Enables Tissue-Specific Metabolic Programs In Vivo.

Ian J Gonzalez1, Aaron D Wolfe1, Benjamin Clark1

  • 1Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine; New Haven, CT 06510, USA.

Biorxiv : the Preprint Server for Biology
|November 26, 2025
PubMed
Summary

Tissue metabolism varies, but how conserved pathways achieve this is unclear. This study reveals glucose 6-phosphate isomerase (GPI-1) has isoform-specific roles in glycolysis and the pentose phosphate pathway (PPP) in vivo.

More Related Videos

Spatial Molecular Imaging of the Glycome Using Mass Spectrometry
08:52

Spatial Molecular Imaging of the Glycome Using Mass Spectrometry

Published on: November 28, 2025

368
Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes
06:26

Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes

Published on: March 28, 2025

916

Related Experiment Videos

Last Updated: Jan 10, 2026

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
08:40

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

Published on: November 21, 2016

30.7K
Spatial Molecular Imaging of the Glycome Using Mass Spectrometry
08:52

Spatial Molecular Imaging of the Glycome Using Mass Spectrometry

Published on: November 28, 2025

368
Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes
06:26

Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes

Published on: March 28, 2025

916

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Metabolism

Background:

  • Metabolic heterogeneity allows tissues to meet diverse physiological demands.
  • The mechanisms underlying tissue-specific metabolic regulation by conserved pathways remain poorly understood, especially in vivo.
  • Glucose 6-phosphate isomerase (GPI-1) is a key enzyme in glycolysis and regulates the pentose phosphate pathway (PPP).

Purpose of the Study:

  • To investigate the tissue-specific functions of GPI-1 in vivo.
  • To elucidate how different GPI-1 isoforms contribute to metabolic heterogeneity.
  • To understand the role of subcellular localization in metabolic regulation.

Main Methods:

  • Utilized Caenorhabditis elegans as a model organism.
  • Employed CRISPR-Cas9 genome editing to create gpi-1 knockout animals.
  • Analyzed tissue-specific expression and subcellular localization of GPI-1 isoforms (GPI-1A and GPI-1B).

Main Results:

  • gpi-1 knockout animals exhibited germline defects (impaired PPP) and somatic defects (impaired glycolysis).
  • Two GPI-1 isoforms, GPI-1A and GPI-1B, showed differential expression and localization.
  • GPI-1A localized to the cytosol in most tissues, while GPI-1B localized to foci near the ER in the germline.
  • GPI-1B expression rescued reproductive fitness but not wild-type glycolytic dynamics.

Conclusions:

  • GPI-1 possesses isoform-specific functions that are critical for tissue-specific metabolism in vivo.
  • Subcellular localization of GPI-1 isoforms contributes to compartmentalized metabolic flux.
  • This study highlights the importance of isoform diversity and spatial compartmentalization in achieving metabolic heterogeneity.