Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neuronal Communication01:28

Neuronal Communication

2.9K
Neurons, the fundamental units of the brain and nervous system, communicate through complex electrochemical signals that underpin all cognitive and bodily functions. This communication is primarily facilitated by a process involving the generation and propagation of an action potential along the axon of the neuron. When the internal electrical charge of a neuron surpasses a certain threshold, an action potential is triggered. This rapid change in voltage travels swiftly along the axon to the...
2.9K
Excitatory and Inhibitory Effects of Neurotransmitters01:29

Excitatory and Inhibitory Effects of Neurotransmitters

12.5K
When an action potential reaches the presynaptic axon terminal, it releases neurotransmitters from the neuron into the synaptic cleft at a chemical synapse. The released neurotransmitter can be excitatory or inhibitory. The critical criteria commonly used to determine whether a molecule is a neurotransmitter at a chemical synapse are the molecule's presence in the presynaptic neuron. Second, its release is in response to strong presynaptic depolarization. And lastly, the presence of...
12.5K
Synaptic Signaling01:12

Synaptic Signaling

79.1K
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
79.1K
Synaptic Signaling01:09

Synaptic Signaling

6.5K
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
6.5K
The Synapse02:47

The Synapse

132.5K
Neurons communicate with one another by passing on their electrical signals to other neurons. A synapse is the location where two neurons meet to exchange signals. At the synapse, the neuron that sends the signal is called the presynaptic cell, while the neuron that receives the message is called the postsynaptic cell. Note that most neurons can be both presynaptic and postsynaptic, as they both transmit and receive information.
132.5K
Electrical Synapses01:28

Electrical Synapses

10.1K
Electrical synapses found in all nervous systems play important and unique roles. In these synapses, the presynaptic and postsynaptic membranes are very close together (3.5 nm) and are actually physically connected by channel proteins forming gap junctions.
Gap junctions allow the current to pass directly from one cell to the next. In contrast, in the chemical synapse, the neurotransmitters carry the information through the synaptic cleft from one neuron to the next. They consist of two...
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developmentally dynamic, non-convergent transcriptomic profiles in CNV models for schizophrenia risk.

Molecular psychiatry·2026
Same author

Human brain prefrontal cortex proteomics identifies compromised energy metabolism and neuronal function in Schizophrenia.

Nature communications·2026
Same author

Genomic analyses reveal new insights into Alzheimer's disease.

medRxiv : the preprint server for health sciences·2025
Same author

Single-cell mRNA-regulation analysis reveals cell type-specific mechanisms of type 2 diabetes.

Nature communications·2025
Same author

Benchmarking methods integrating GWAS and single-cell transcriptomic data for mapping trait-cell type associations.

medRxiv : the preprint server for health sciences·2025
Same author

Transcriptomic and genetic analysis suggests a role for mitochondrial dysregulation in schizophrenia.

medRxiv : the preprint server for health sciences·2025
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jan 10, 2026

Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo
10:19

Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo

Published on: March 31, 2016

8.5K

Cell Type Specific Inference of Perturbations in Synaptic Communication with MultiNeuronChat.

Gianluca Volkmer1, Jens Hjerling-Leffler1, Lisa Bast1

  • 1Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden.

Biorxiv : the Preprint Server for Biology
|November 26, 2025
PubMed
Summary
This summary is machine-generated.

MultiNeuronChat enhances cell communication analysis from single-nucleus RNA sequencing data. This framework precisely identifies altered neuron-neuron and neuron-glia communication pathways at the cell type pair level, aiding disease research.

More Related Videos

Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond
08:08

Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond

Published on: June 24, 2015

12.0K
Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
08:38

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Published on: May 25, 2011

16.0K

Related Experiment Videos

Last Updated: Jan 10, 2026

Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo
10:19

Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo

Published on: March 31, 2016

8.5K
Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond
08:08

Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond

Published on: June 24, 2015

12.0K
Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
08:38

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Published on: May 25, 2011

16.0K

Area of Science:

  • Neuroscience
  • Genomics
  • Computational Biology

Background:

  • Single-nucleus RNA sequencing (snRNA-seq) enables studying cellular communication.
  • Current methods lack cell type pair resolution for differential communication analysis.
  • Understanding altered cell communication is crucial for disease and developmental studies.

Purpose of the Study:

  • To develop a high-resolution framework for inferring differential neuron-neuron and neuron-glia communication.
  • To enable analysis at the cell type pair level using snRNA-seq data.
  • To improve biological and medical insights from communication pathway alterations.

Main Methods:

  • MultiNeuronChat framework integrating snRNA-seq gene expression data.
  • Utilizes a comprehensive database of cell adhesion molecules, gap junctions, and synaptic transmission.
  • Performs differential analysis of cell communication at the cell type pair level.

Main Results:

  • Accurate and efficient prediction of cell type-specific perturbations in silico.
  • Identification of known and novel communication pathways in Alzheimer's disease patient data.
  • Demonstrated sensitivity in detecting subtle communication changes.

Conclusions:

  • MultiNeuronChat provides a highly resolved method for analyzing cell communication from snRNA-seq data.
  • The framework enhances understanding of cell type-specific communication alterations in disease contexts.
  • Donor-specific communication scores can inform patient stratification and personalized medicine.